| 楸树扦插过程中插穗内含物变化分析 |
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| 引用本文： | 王改萍,王晓聪,章雷,张晨,刘彬.楸树扦插过程中插穗内含物变化分析[J].浙江农林大学学报,2021,38(2):296-303. |
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| 作者姓名： | 王改萍 王晓聪 章雷 张晨 刘彬 |
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| 作者单位： | 1.南京林业大学 林学院，江苏 南京 2100372.南京林业大学 南方现代林业协同创新中心，江苏 南京 210037 |
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| 基金项目： | “十三五”国家重点研发计划资助项目(2017YFD06000604)。 |
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| 摘 要： | 目的 探索楸树Catalpa bungei扦插生根机制，研究插穗生根过程的形态特征和生理生化特性。 方法 采用ABT生根粉1号(GGR-6)浸泡处理楸树插穗，以蒸馏水浸泡为对照，测定扦插生根不同阶段相关酶活性、营养物质及生根抑制物质的变化规律。 结果 GGR-6处理显著促进插穗生根，生根率达到82.04%，高于对照(P＜0.01)。生根过程中，超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)活性在大量愈伤组织形成期均上升；SOD、PPO最大值出现在不定根大量形成期，分别为623.33×16.67和57.44×16.67 nkat·g?1，POD在不定根大量形成期下降；3种酶均与生根率成显著正相关。插穗可溶性糖、碳氮比(C/N)在大量愈伤组织形成期急剧下降，仅为扦插初时的40%左右，扦插20 d时达到最大值。可溶性蛋白质、总氮质量分数总体呈下降趋势，25~30 d时为最小值；GGR-6处理下插穗内营养物质变化幅度大，且最大值均高于对照；总氮质量分数与生根率负相关(P＜0.05)，可溶性糖、可溶性蛋白质、C/N均与生根率显著正相关(P＜0.05)；气相色谱质谱联用仪(GC-MS)鉴定出4种与生根相关的物质，其中3种可能抑制生根，1种可能促进生根。 结论 插穗抗氧化酶活性的提高促进了楸树不定根的发生；可溶性糖与可溶性蛋白质是插穗生根的主要营养物质；初步推断槲皮素、亚油酸、棕榈酸抑制插穗生根，植物甾醇促进插穗生根。图2表4参32
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| 关 键 词： | 楸树 扦插 相关酶 营养物质 生根抑制物 |
| 收稿时间： | 2020-05-13 |
Cutting test of Catalpa bungei and change analysis of cutting contents |
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| WANG Gaiping,WANG Xiaocong,ZHANG Lei,ZHANG Chen,LIU Bin.Cutting test of Catalpa bungei and change analysis of cutting contents[J].Journal of Zhejiang A&F University,2021,38(2):296-303. |
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| Authors: | WANG Gaiping WANG Xiaocong ZHANG Lei ZHANG Chen LIU Bin |
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| Institution: | 1.College of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China2.Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, Jiangsu, China |
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| Abstract: | Objective]The objective of this study is to explore the rooting mechanism of Catalpa bungei,as well as the morphological characteristics and physiological and biochemical characteristics of cuttings during rooting process.Method]The cuttings of C.bungei were treated with dissolved GGR-6 to promote rooting,and the enzyme activities,nutrients and rooting inhibitors in different rooting stages were measured.Result]GGR-6 treatment significantly promoted the rooting of cuttings,and the rooting rate reached 82.04%,higher than that of the control treatment(P<0.01).During the rooting process,the activities of superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO)increased during the formation of a large number of callus,and the maximum values of SOD and PPO were 623.33×16.67 and 57.44×16.67 nkat·g-1,respectively.POD activity decreased during the formation of adventitious roots,and the three enzymes were positively correlated with the rooting rate.The soluble sugar and C/N ratio in cuttings decreased sharply at the callus stage,only about 40%of the initial value,and reached the maximum at 20 days of cutting.The soluble protein and total nitrogen showed a decreasing trend,and reached the minimum at 25-30 days.Under GGR-6 treatment,the change range of nutrients in cuttings was large,and the maximum values were higher than those of the control.Total nitrogen content was negatively correlated with rooting rate(P<0.05),while soluble sugar,soluble protein and C/N were significantly positively correlated with rooting rate(P<0.05).GC-MS analysis identified four rooting related substances,three of which might inhibit rooting and one might promote rooting.Conclusion]The increase of antioxidant enzyme activities of cuttings can promote the occurrence of adventitious roots of C.bungei.Soluble sugar and soluble protein are the main nutrients of cuttings.Quercetin,linoleic acid and palmitic acid may inhibit the rooting of cuttings and phytosterol may promote the rooting of cuttings. |
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| Keywords: | Catalpa bungei cuttings enzymes nutrients rooting inhibitor |
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