| 生物炭对小麦根际和根内微生物群落结构的影响 |
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| 引用本文： | 徐民民,黄莹,李波,徐艳,张帅,姚岭芸,王政.生物炭对小麦根际和根内微生物群落结构的影响[J].浙江农业学报,2021,33(3):516. |
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| 作者姓名： | 徐民民 黄莹 李波 徐艳 张帅 姚岭芸 王政 |
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| 作者单位： | 1. 山东省环境保护科学研究设计院有限公司,山东 济南 2501002.青岛大学 环境科学与工程学院,山东 青岛 266071 |
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| 基金项目： | 山东省自然科学基金(ZR2019YQ18);山东省高等学校青创人才引育计划(DC2000000961) |
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| 摘 要： | 为了研究生物炭对小麦根际和根内微生物群落组成的影响,开展盆栽培养试验,利用高通量测序技术分析生物炭对小麦根内、根际和空白土壤中细菌群落结构组成的影响。结果表明,小麦根内微生物的群落多样性显著(P<0.05)低于根际和空白土壤中的微生物群落多样性,即植物根系能够过滤和筛选部分微生物在其根内定殖生长,其中,变形菌门(Proteobacteria)和蓝藻门(Cyanobacteria)是小麦根内环境中的优势菌门。生物炭能够显著(P<0.05)影响小麦根内和根际微生物组的结构组成。在根内,加生物炭显著(P<0.05)提高了疣微菌科(Verrucomicrobiaceae)和Luteolibacter属细菌的相对丰度;在根际,加生物炭条件下相对丰度显著(P<0.05)上升的微生物菌群包括疣微菌门(Verrucomicrobia)、厚壁菌门(Firmicutes)、芽单胞菌门(Gemmatimonadetes)、芽孢杆菌目(Bacillales)、环脂酸芽孢杆菌科(Alicyclobacillaceae)、Luteolibacter、Tumebacillus、芽单胞菌属(Gemmatimonas)、小坂菌属(Kosakonia)、溶杆菌属(Lysobacter)、假黄色单胞菌属(Pseudoxanthomonas)、Blastomonas、马赛菌属(Mssilia)、原囊菌属(Archangium)和肠杆菌属(Enterobacter)。整体来看,根际微生物群落对生物炭处理更为敏感,生物炭添加会显著(P<0.05)影响小麦根际的标志微生物种类。
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| 关 键 词： | 小麦 生物炭 高通量测序 微生物多样性 |
| 收稿时间： | 2020-08-17 |
Effect of biochar on wheat root-associated microbial community structures |
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| XU Minmin,HUANG Ying,LI Bo,XU Yan,ZHANG Shuai,YAO Lingyun,WANG Zheng.Effect of biochar on wheat root-associated microbial community structures[J].Acta Agriculturae Zhejiangensis,2021,33(3):516. |
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| Authors: | XU Minmin HUANG Ying LI Bo XU Yan ZHANG Shuai YAO Lingyun WANG Zheng |
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| Institution: | 1. Shandong Academy of Environmental Sciences Co., Ltd., Jinan 250100, China
2. College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China |
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| Abstract: | In order to study the effect of biochar on the wheat root-associated microbiome, greenhouse experiment was conducted and the microbial communities in wheat endosphere, rhizosphere and bulk soil were sequenced by high throughput sequencing technology. The results showed that the microbial community diversity in endosphere was significantly (P<0.05) lower than that in the rhizosphere and bulk soil. In other words, plant roots could filter and screen some microorganisms to colonize and grow in the roots, among which Proteobacteria and Cyanobacteria were the dominant bacteria. Biochar could influence the structure and composition of wheat root-associated microbial community. In the endosphere, the relative abundance of Verrucomicrobiaceae and Luteolibacter was significantly (P<0.05) increased with biochar addition; while in the rhizosphere, biochar addition significantly (P<0.05) increased the relative abundance of Verrucomicrobia, Firmicutes, Gemmatimonadetes, Bacillales, Alicyclobacillaceae, Luteolibacter, Tumebacillus, Gemmatimonas, Kosakonia, Lysobacter, Pseudoxanthomonas, Blastomonas, Mssilia, Archangium and Enterobacter. The microbial communities in the rhizosphere was more sensitive to biochar, and the addition of biochar significantly (P<0.05) changed the biomarkers in the rhizosphere of wheat. |
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| Keywords: | wheat biochar high throughput sequencing microbial diversity |
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