Effect ofl-phenylalanine on rooting of shoots from axillary buds of adultLarix leptolepis |
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| Authors: | Shinjiro Ogita Takafumi Kubo Masami Fushitani |
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| Institution: | (1) Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, 183 Tokyo, Japan |
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| Abstract: | Rooting of shoots derived from axillary buds was examined to establish an efficient shoot culture system of clonal micropropagation
in adult tree ofLarix leptolepis Gord. (Japanese larch). Nine out of ten shoots induced calli (90%) on their shoot bases, and the two of them formed root
primordia with a red pigment (20%) on the calli surface within 5 weeks after culturing on modified Murashige and Skoog (MS)
medium supplemented with 1.5 μM of indolebutyric acid (IBA) and 1.5 μM of naphthaleneacetic acid (NAA). However, the primordia
did not elongate actively. The addition of 10 mMl-phenylalanine in the MS medium with the auxins resulted in the formation of roots at high frequency, about 80%, and they
elongated actively. Although callus was formed in all the shoots cultured on the medium withl-phenylalanine, it appeared that the callus development was less as compared to the medium withoutl-phenylalanine. Consequently, the rooting might be associated with the suppression of the induced callus. |
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| Keywords: | adult tree Larix leptolepis l-phenylalanine" target="_blank">l-phenylalanine root formation |
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