| 军曹鱼幼鱼维生素C需要量的研究 |
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| 引用本文： | 赵红霞,曹俊明,谭永刚,梁海鸥,吴建开,杨大伟.军曹鱼幼鱼维生素C需要量的研究[J].动物营养学报,2008,20(4). |
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| 作者姓名： | 赵红霞 曹俊明 谭永刚 梁海鸥 吴建开 杨大伟 |
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| 作者单位： | 1. 广东省农业科学院畜牧研究所,广州,510640
2. 广东恒兴集团有限公司,湛江,524000 |
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| 摘 要： | 选用初体均重为25.85 g的军曹鱼幼鱼225尾随机分为5组,每组设3个重复,每个重复15尾.分别投喂添加5种不同剂量维生素C(添加量分别为0、37.5、75、150和300 mg/kg)的试验饲料,试验期为56 d,观察维生素c对军曹鱼幼鱼生长、组织维生素C积累量及非特异性免疫的影响.结果表明,随着饲料中维生素C添加量增加,军曹鱼幼鱼增重率和特定生长率呈现出上升后下降趋势,在75 mg/kg时达到最高值,显著高于0和300 mg/kg组(P<0.05).全鱼粗蛋白质含量随着饲料中维生素C添加量的升高亦呈现先上升后下降趋势,在75 mg/kg时达到最高值,全鱼干物质、粗蛋白质、粗脂肪、灰分含量在各组之间均无显著差异(P>0.05).血清维生素C积累量随着饲料中维生素C添加量的增加呈现先上升后下降趋势,在150 mg/kg时达到最高值且显著高于0 mg/kg组(P<0.05);脑和肝脏中维生素C含量在300 mg/kg时达到最高值.300 mg/kg组脑中维生素C含量显著高于0,37.5和75 mg/kg组(P<0.05),肝脏中维生素C含量显著高于0,37.5和150 mg/kg组(P<0.05).随着饲料中维生素C添加量的增加,溶菌酶活性呈现先上升后下降趋势,在75 mg/kg达到最高,显著高于0,37.5和300 mg/kg组(P<0.05);血清总抗氧化能力呈现上升趋势,75、150和300 mg/kg组显著高于0 mg/kg组(P<0.05);肾抗氧离子自由基在150 mg/kg组达到最高,但各组之间无显著差异(P>0.05).以鱼体增重率和血清溶菌酶含量为指标,经折线模型回归分析求得军曹鱼幼鱼饲料中维生素C的适宜添加量分别为70和80.72 mg/kg.
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| 关 键 词： | 军曹鱼 维生素C 生长 维生素C积累 非特异性免疫 |
Study on Vitamin C Requirement of Juvenile Cobia (Rachycentron canadum L. ) |
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| ZHAO Hong-xia,GAO Jun-ming,TAN Yong-gang,LIANG Hai-ou,WU Jian-kai,YANG Da-wei.Study on Vitamin C Requirement of Juvenile Cobia (Rachycentron canadum L. )[J].Acta Zoonutrimenta Sinica,2008,20(4). |
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| Authors: | ZHAO Hong-xia GAO Jun-ming TAN Yong-gang LIANG Hai-ou WU Jian-kai YANG Da-wei |
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| Abstract: | The experiment was carried out to determine the dietary vitamin C requirement of juvenile Cobia(Rachycentron canadum L.).Two hundred and twenty five fish with an average body weight of 25.85 g were randomly divided into 5 groups with 3 replicates and each replicate contained 15 fish;Five test forages containing different doses of vitamin C(0,37.5,75,150,300 mg/kg) were used to feed the fish for 56 days.The optimal dietary vitamin C requirement was evaluated based on growth performance,vitamin C accumulation contents in tissues and non-specific immunity in cobia juveniles.The results indicated that fish fed with 75 mg/kg of vitamin C had highest weight gain rate(WGR) and special gain rate(SGR),which were significantly higher than that of 0 and 300 mg/kg group(P<0.05).With the increase of dietary vitamin C,the crude protein content of whole fish increased at first and then decreased,which reached a peak at 75 mg/kg.But in each group,no significant difference was obtained in the content of dry matter,crude protein,crude fat and crude ash of whole body(P>0.05).With the increase of the level of dietary vitamin C,vitamin C accumulations of serum in fish increased and reached a peak,and then decreased,and the highest vitamin C accumulations of serum were found in the fish fed with 150 mg/kg of vitamin C,which was significantly higher that of 0 mg/kg group(P<0.05).The vitamin C accumulations in liver and brain of fish fed with 300 mg/kg of vitamin C were the highest.The vitamin C accumulations in the brain of 300 mg/kg group were significantly higher than that of 0,37.5 and 75 mg/kg groups(P<0.05),and the vitamin C accumulations in the liver were significantly higher than that of 0,37.5 and 150 mg/kg groups(P<0.05).Lysozyme increased at first and then decreased with the increase of the level of dietary vitamin C,and the lysozyme in 75 mg/kg group was the highest,which was significantly higher than that of 0,37.5 and 300 mg/kg groups.Total antioxidant competence(T-AOC) levels increased significantly when the levels of dietary vitamin C increased from 0 to 300 mg/kg,and 75,150,300 mg/kg groups were significantly higher than 0 mg/kg group(P<0.05).The highest renal antioxygen ion free radicals were found in the fish fed with 150 mg/kg of vitamin C,but no significant difference was obtained in each group(P>0.05).Set the weight gain ratio of the fish and the serum lysozyme content as the indices,the regression analysis of turning curve model showed that the optimal vitamin C requirement for juvenile cobia growth was 70 and 80.72 mg/kg respectively. |
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| Keywords: | Cobia(Rachycentron canadum L ) Vitamin C Growth Vitamin C accumulations Non-specific immunity |
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