| LAMP检测沙门氏菌方法的建立 |
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| 引用本文： | 洪艳,贺凡,王晓闻.LAMP检测沙门氏菌方法的建立[J].山西农业科学,2014(4):340-342. |
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| 作者姓名： | 洪艳 贺凡 王晓闻 |
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| 作者单位： | [1]山西农业大学文理学院,山西太谷030801 [2]山西农业大学食品科学与工程学院,山西太谷030801 |
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| 摘 要： | 环介导等温扩增(LAMP)是利用设计的4条特殊引物和具有链置换活性的DNA聚合酶,在恒温条件下特异、高效、快速地扩增DNA的新技术。研究选取沙门氏菌编码DNA解旋酶B亚单位的gyrB基因设计了1套引物,总反应体系25μL,63℃恒温1 h,用LAMP对9株沙门氏菌属扩增的结果均为阳性,而大肠杆菌O216等7株致病菌株扩增的结果均为阴性;LAMP的灵敏度为6.8×101cfu/mL。LAMP检测方法更快速、灵敏,有着较为广泛的发展前景。
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| 关 键 词： | 沙门氏菌 LAMP 灵敏度 特异性 |
Detection of Salmonella by Loop-mediated Isothermal Amplification Method |
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| HONG Yan,HE Fan,WANG Xiao-wen.Detection of Salmonella by Loop-mediated Isothermal Amplification Method[J].Journal of Shanxi Agricultural Sciences,2014(4):340-342. |
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| Authors: | HONG Yan HE Fan WANG Xiao-wen |
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| Institution: | 1.College of Arts ＆ Sciences, Shanxi Agricultural University, Taigu 030801, China; 2.College of Food Science ＆ Engineering, Shanxi Agricultural University, Taigu 030801, China) |
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| Abstract: | Loop-mediated isothermal amplification （LAMP） is a new technology which could amplify DNA specifically, efficiently and fast in the constant temperature conditions by four specially designed primers and a chain displacement activity of DNA polymerase. One pair of primer was designed depending on Salmonella gyrB gene sequence which have been reported in genbank. The reaction was run under 63℃ for 1 hour. The amplication result of 9 Salmonella strains were positive and the rest 7 E. coli O216 negative; With sensitivity of 6.8 × 10^1 euf/mL, the detecting way by LAMP is more fast and sensitive and has a brighter development prospect. |
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| Keywords: | Salmonella LAMP detection specificity |
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