| 采用PCR方法快速检测食品中的蛔虫卵 |
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| 引用本文： | 赵昕,郑秋月,田苗,曹际娟.采用PCR方法快速检测食品中的蛔虫卵[J].大连水产学院学报,2009(Z1). |
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| 作者姓名： | 赵昕 郑秋月 田苗 曹际娟 |
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| 作者单位： | 辽宁出入境检验检疫局; |
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| 摘 要： | 根据蛔虫的特异性基因序列,设计了PCR特异性引物,并进行灵敏性和特异性试验。结果表明:用所建立的PCR检测食品中的蛔虫卵,特异性强,检测灵敏度高,可检测到食品中的一个虫卵。
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| 关 键 词： | 食品 蛔虫卵 PCR |
Detection of eggs in round worm Ascaris lumbricoides by PCR |
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| ZHAO Xin,ZHENG Qiu-yue,TIAN Miao,CAO Ji-juan.Detection of eggs in round worm Ascaris lumbricoides by PCR[J].Journal of Dalian Fisheries University,2009(Z1). |
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| Authors: | ZHAO Xin ZHENG Qiu-yue TIAN Miao CAO Ji-juan |
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| Institution: | ZHAO Xin,ZHENG Qiu-yue,TIAN Miao,CAO Ji-juan(Liaoning Entry-Exit Inspection , Quarantine Bureau,Dalian 116001,China) |
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| Abstract: | A method for detection of round worm Ascaris lumbricoides eggs in food was developed by a quick,sensitive and special PCR method.The primers of PCR were designed and synthesized based on the specific sequence of the round worm.The designed primers were found to have high specificity,and it was proved that the methods had high sensitivity. |
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| Keywords: | food Ascaris lumbricoides Linnaeus PCR |
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