| 滇一型杂交粳稻恢复基因的分子鉴定研究 |
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| 引用本文： | 谭亚玲,谭学林.滇一型杂交粳稻恢复基因的分子鉴定研究[J].分子植物育种,2004,2(2):209-214. |
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| 作者姓名： | 谭亚玲 谭学林 |
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| 作者单位： | 云南农业大学稻作研究所,昆明,650201 |
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| 基金项目： | 本研究由云南省科技厅重点基金资助(1999C005Z). |
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| 摘 要： | 用位于水稻第10染色体的微卫星标记OSR33、RM228对285份滇型材料进行恢复基因的分子鉴定研究，结果表明：①OSR33、RM228标记的基因型依材料的不同而出现分子量不同的带型，且带型与材料有关；②OSR33和RM228鉴定材料的准确率分别为96％和90％；OSR33、RM228标记的基因型值与黑染花粉率极显著正相关，可用于鉴别恢复系；③用Mapmaker／QTL软件分析供试材料的表现型，在OSR33、RM228之间，探测到1个与育性恢复有关的主效QTL，可解释83．2％的表型变异，与OSR33和RM228的遗传距离分别为3．3cM和7．0cM。
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| 关 键 词： | 滇一型杂交粳稻 恢复基因 分子鉴定 恢复系 分子标记辅助选择 |
Molecular Identification of the Fertility-restorer-gene of Dian-type 1 Hybrid Rice |
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| Tan Yaling Tan Xuelin Rice Research Institute of Yunnan Agricultural University,Kunming.Molecular Identification of the Fertility-restorer-gene of Dian-type 1 Hybrid Rice[J].Molecular Plant Breeding,2004,2(2):209-214. |
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| Authors: | Tan Yaling Tan Xuelin Rice Research Institute of Yunnan Agricultural University Kunming |
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| Institution: | Tan Yaling * Tan Xuelin Rice Research Institute of Yunnan Agricultural University,Kunming,650201 |
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| Abstract: | The identification of fertility-restoring-gene of Dian-type 1 hybrid rice with two simple sequence repeat(SSR) markers, OSR33 and RM228 locating in the middle of the long arm of chromosome 10, were studied. The correct frequency of the identification was 95% with OSR33, and 90% with RM228. Statistics analysis showed very significant correlation between pollen fertility and marker genotypes. The results indicated that the two markers could be used for identifying restorer lines of Dian type rice. A major-fertility-restoring-gene was detection between OSR33 and RM228 by QTL mapping approach. The locus was closed to markers OSR33 and RM228 within 3.3cM and 7.0cM respectively, which explained 83.2% phenotypic variance. |
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| Keywords: | Dian-type 1 hybrid rice Fertility-restoring-gene Molecular identification |
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