| 利用基因枪法向高羊茅导入P5CS基因的研究 |
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| 引用本文： | 李志亮,黄丛林,张秀海,曹鸣庆,李征,王刚,吴忠义.利用基因枪法向高羊茅导入P5CS基因的研究[J].园艺学报,2005,32(4):853-657. |
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| 作者姓名： | 李志亮 黄丛林 张秀海 曹鸣庆 李征 王刚 吴忠义 |
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| 作者单位： | ( 北京农业生物技术研究中心, 北京100089; 北京草业与环境研究发展中心, 北京100089; 河北师范大学生命科学学院, 石家庄050016) |
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| 基金项目： | 北京市基金重点项目(6001001)，北京市科委项目(H020821330130,H012010240240113)，北京市优秀人才项目(20041D0200504) |
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| 摘 要： | 利用基因枪法, 以豇豆(V igna aconitifolia) 的Δ1 - 吡咯啉- 5 - 羧酸合成酶基因( P5CS ) 的突变型P5CS-F129A基因转化高羊茅的下胚轴愈伤组织, 获得25株经PCR检测和Southern blot分析为阳性的转基因植株, 转化频率约为1.4%。脯氨酸含量测定表明, 转基因植株的脯氨酸含量比对照高31% ～83%。对植株的抗旱性初步检测表明, 转基因植株比对照耐旱。
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| 关 键 词： | 草坪草 高羊茅 转基因 基因枪 耐旱性 |
| 文章编号： | 0513-353X（2005）04-0653-05 |
| 收稿时间： | 2004-09-11 |
| 修稿时间： | 2004-09-112005-01-17 |
Studies on Transferring P5CS Gene into Tall Fescue(Festuca arundinacea Schreb.)via Microprojectile Bombardment |
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| Li Zhiliang,Huang Conglin,Zhang Xiuhai,Cao Mingqing,Li Zheng,Wang Gang,Wu Zhongyi.Studies on Transferring P5CS Gene into Tall Fescue(Festuca arundinacea Schreb.)via Microprojectile Bombardment[J].Acta Horticulturae Sinica,2005,32(4):853-657. |
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| Authors: | Li Zhiliang Huang Conglin Zhang Xiuhai Cao Mingqing Li Zheng Wang Gang Wu Zhongyi |
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| Institution: | (Beijing Research Center of Agro-Biotechnology, Beijing 100089, China; Beijing Research and Development Center for Grass and Environment, Beijing 100089, China; College of Life Science Hebei Normal University, Shijiazhuang 050016, China) |
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| Abstract: | In this study, a mutated form of V igna aconitifolia Δ1-pyrroline-5-carboxylate synthetase( P5CS -F129A ) gene was transferred into calli of tall fescue by micro p rojectile bombardment. Twenty2five transgenic p lants were obtained through phosphinothricin screening, PCR and Southern blot analysis. Thetransformation frequency in calli of tall fescue was about 1.4%. The content of proline in transgenic plants was 31% - 83% higher than that in untransformed plants. Furthermore, the transgenic p lants were more tolerant to drought stress. |
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| Keywords: | Turfgrass Tall fescue Transgenic Micro projectile bombardment Drought tolerance |
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