| TTC法测定山豆根种子生活力条件的优化 |
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| 引用本文： | 马洪娜,吴依琳,何雨,檀龙颜,秦睿.TTC法测定山豆根种子生活力条件的优化[J].种子,2019(9):43-47. |
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| 作者姓名： | 马洪娜 吴依琳 何雨 檀龙颜 秦睿 |
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| 作者单位： | 贵州中医药大学药学院;紫云自治县长萌农业科技开发有限责任公司 |
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| 基金项目： | 国家自然科学基金(31860056);贵州省科技支撑计划项目(黔科合支撑[2018]2798);贵州省基础研究计划项目(黔科合基础[2019]1034号) |
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| 摘 要： | 以山豆根种子为实验材料,采用TTC法测定生活力,分别通过正交试验、单因素试验和Central Composite Design响应面试验对测定条件进行优化,从TTC浓度、染色温度和染色时间为指标。结果显示,各因素对种子生活力测定的影响差异不显著;单因素试验的最佳测试条件为TTC浓度0.7%、染色温度40 ℃、染色时间4 h;Central Composite Design响应面试验优化后,最佳测试条件与单因素试验结果一致。表明用响应面分析法对TTC法测定山豆根种子生活力的条件进行优化是可靠的。
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| 关 键 词： | 山豆根 种子生活力 TTC法 响应面分析 |
Optimization of Seed Vigor Test Conditions by in TTC Method in Sophora Tonkinensis |
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| MA Hongna,WU Yilin,HE Yu,TAN Longyan,QIN Rui.Optimization of Seed Vigor Test Conditions by in TTC Method in Sophora Tonkinensis[J].Seed,2019(9):43-47. |
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| Authors: | MA Hongna WU Yilin HE Yu TAN Longyan QIN Rui |
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| Institution: | (School of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China;Ziyun Autonomous County Changmeng Agricultural Science and Technology Development Co., Ltd., Anshun Guizhou 561000, China) |
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| Abstract: | In this paper, the seed vitality of the Sophora tonkinensis seeds was determined by TTC method. The measurement conditions were optimized through orthogonal test, single factor test and Central Composite Design response surface test, respectively, and the indexes of TTC concentration, dyeing temperature and dyeing time were also measured. The results showed that there was no significant difference in the effect of different factors on seed vigor. The optimal test conditions of single factor test were TTC concentration of 0.7%, dyeing temperature of 40 ℃ and dyeing time of 4 h, while the same results were also found in Central Composite Design response surface test after optimization. These results showed that the response surface analysis wer reliable to optimize the condition of TTC method for determining seed vigor in sophora tonkinensis. |
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| Keywords: | Sophora tonkinensis seed viability TTC method response surface analysis |
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