| 水稻花色苷含量及合成结构基因的差异表达研究 |
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| 引用本文： | 袁昭弟,焦后意,刘凯,周丽洁,余显权.水稻花色苷含量及合成结构基因的差异表达研究[J].种子,2019(9):11-17. |
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| 作者姓名： | 袁昭弟 焦后意 刘凯 周丽洁 余显权 |
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| 作者单位： | 贵州大学农学院;贵州大学水稻研究所 |
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| 基金项目： | 国家自然科学基金“贵州水稻地方品种资源食味相关品质的关联分析与优异品质资源挖掘”(31660383);贵州省农业科技支撑计划项目“贵州省特色优质稻品种(系)的选育”(黔科合支撑[2019]2302号)共同支助 |
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| 摘 要： | 水稻花色苷的生物合成受许多因素影响,而控制关键酶的结构基因是最主要的因素。针对2个红米材料和2个白米材料,采用实时荧光定量的方法,对合成水稻花色苷的结构基因( OsPAL 、 OsCHS 、 OsCHI 、 OsF3H 、 OsF3′H 、 OsDFR 、 OsANS 、 OsF3′5′H 、 OsUFGT )在茎、叶及种子发育过程中的相对表达水平进行了测定。结果表明:1) OsPAL 、 OsCHS 在水稻的茎、叶和种子中表达, OsCHI 在白米恢复系蜀恢498和白米株系的组织中均有表达,是花色苷合成的组成型基因;其它基因在参试材料的茎、叶和种子中的表达量不同,具有组织特异性。2)在红米品种贵红1号和红米株系开花后7~28 d的花色苷含量逐渐增加,而白米恢复系蜀恢498和白米株系的花色苷含量逐渐下降;这些基因在红米品种贵红1号和红米株系的前中后期均有表达且表达量较高, OsCHS 在红米品种贵红1号种子发育过程中的表达量逐渐增加;这些基因在白米恢复系蜀恢498和白米株系开花后7~28 d的前中期表达量较低,后期急剧降低。表明这些基因的表达量与其花色苷的含量动态变化相同,对花色苷的积累有一定的作用。3) OsPAL 、 OsCHS 表达量与红米品种贵红1号花色苷含量呈显著正相关, OsF3H 表达量与白米株系花色苷含量呈显著正相关, OsPAL 表达量与红米株系花色苷含量呈显著正相关, OsCHI 、 OsF3′5′H 、 OsUFGT 表达量与红米株系花色苷含量呈极显著正相关。研究表明,这些基因在参试材料的茎、叶和种子开花后7~28 d前中后期的差异表达,可能是造成它们着色差异的主要原因。 OsPAL 、 OsCHS 是红米品种贵红1号籽粒花色苷合成的关键基因, OsF3H 在白米株系籽粒发育过程中扮演重要的角色, OsPAL 、 OsCHI 、 OsF3′5′H 、 OsUFGT 在红米株系籽粒花色苷积累中起重要作用。
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| 关 键 词： | 水稻 花色苷含量 荧光定量 结构基因 表达分析 |
Study on the Change of Anthocyanin Content and Different Expression of Synthetic Structural Genes in Rice |
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| YUAN Zhaodi,JIAO Houyi,LIU Kai,ZHOU Lijie,YU Xianquan.Study on the Change of Anthocyanin Content and Different Expression of Synthetic Structural Genes in Rice[J].Seed,2019(9):11-17. |
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| Authors: | YUAN Zhaodi JIAO Houyi LIU Kai ZHOU Lijie YU Xianquan |
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| Institution: | (College of Agriculture,Guizhou University,Guiyang 550025, China;Research Institute of Rice,Guizhou University,Guiyang 550025, China) |
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| Abstract: | The biosynthesis of rice anthocyanins is influenced by many factors, among which the key enzymes encoded by structural genes are the most important. The relative expression levels of the structural genes (e.g. OsPAL, OsCHS, OsCHI, OsF3H, OsF3′H, OsDFR, OsANS, OsF3′5′H) involved in rice anthocyanin synthesis were studied by Real-time PCR method in stems, leaves and seeds of two red rice varieties and two white rice varieties. The results showed that (1) OsPAL and OsCHS were expressed in stem, leaf and seed of rice, and OsCHI was expressed in the tissues of both white rice restorer line “Shuhui 498” and another white rice lines, which was the constitutive expression gene. The expression of other genes in stem, leaf and seed was different with the characteristics of tissue specificity;After flowering, the anthocyanin content of red rice varieties “Guihong 1” and red rice lines increased gradually from 7 to 28 days, while the anthocyanin content of restorer lines “Shuhui 498” and white rice lines decreased gradually. There was higher expression level of the structural gene in the early, middle and late stages of red rice variety “Guihong 1” and red rice lines, and the expression of OsCHS in the process of seed development of red rice variety “Guihong 1” increased gradually. The expression of these genes in “Shuhui 498” and white rice restorer lines were low in the early and middle stages after 7-28 d after flowering, and it decreased sharply in the late stage, which indicated the expression of structural genes was the same as the change of anthocyanins content, and the accumulation of anthocyanins played a certain role. The expression levels of OsPAL and OsCHS were significantly positively correlated with the anthocyanin content in red rice “Guigong 1”, while OsF3H was significantly positively correlated with the anthocyanin content of white rice lines. Additionally, OsPAL was significantly positively correlated with the anthocyanin content of red rice, while OsCHI, OsF3′5′H and OsUFGT were significantly positively correlated with the anthocyanin content of red rice lines. Studies showed that the differential expression of the structural genes in stems, leaves and seeds at middle and late stage 7-28 days after flowering might be a main reason for the difference in their coloration. OsPAL and OsCHS were the key genes of anthocyanin synthesis in red rice variety “Guihong 1”, while OsF3H played an important role in the development of white rice lines. In a word, OsPAL, OsCHI, OsF3′5′H and OsUFGT played an important role in the accumulation of anthocyanin in red rice lines. |
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| Keywords: | rice anthocyanin content Real-time PCR structural genes expression analysis |
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