| 不同抗冷级别的棉苗低温诱导蛋白比较 |
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| 引用本文： | 李锐,李生泉,范月仙.不同抗冷级别的棉苗低温诱导蛋白比较[J].棉花学报,2010,22(3):254-259. |
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| 作者姓名： | 李锐 李生泉 范月仙 |
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| 作者单位： | 山西农业大学,太谷,030801 |
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| 基金项目： | 山西省自然科学基金项目(20021084) |
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| 摘 要： | 通过蛋白质双向电泳和质谱技术,对低温前后不同抗冷级别的棉花品种锦3047和410的蛋白质组进行了比较分析。结果表明:⑴不同品种的棉苗蛋白质组有明显差异,锦3047在pH4~7条件下的棉苗子叶蛋白表达图谱中发现50个蛋白点在低温胁迫前后的表达量有明显差异。⑵其中30个点在低温前后均有表达,低温处理后,有19个蛋白点的丰度值变大,且抗冷性较强的锦3047绝对丰度明显高于抗冷性较弱的410;11个蛋白点的丰度值变小,锦3047的绝对丰度低于410。⑶锦3047特有8个蛋白点表达量上升,而在410中几乎不表达。⑷对入选的锦3047的差异蛋白点进行质谱分析,并通过数据库检索进行蛋白质鉴定,得到了其中30个蛋白的完整肽指纹图谱。其中逆境蛋白占20%,这些蛋白的表达可能对锦3047的抗冷性具有重要作用。
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| 关 键 词： | 棉花 抗冷性 低温诱导蛋白 |
| 收稿时间： | 2009-11-29; |
Comparison of Low Temperature-induced Protein in Cotton Seedlings with Different Chilling Resistance |
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| LI Rui,LI Sheng-quan,FAN Yue-xian.Comparison of Low Temperature-induced Protein in Cotton Seedlings with Different Chilling Resistance[J].Cotton Science,2010,22(3):254-259. |
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| Authors: | LI Rui LI Sheng-quan FAN Yue-xian |
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| Institution: | Shanxi Agricultural University/a>;Taigu/a>;Shanxi 030801/a>;China |
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| Abstract: | By using two-dimensional electrophoresis and mass spectrometry techniques,the proteomes in different chilling-resistant levels cotton Jin3047(It has obvious chilling resistance) and 410(It has less chilling resistance) before and after low temperature treatments were comparatively analyzed.Results showed that the proteomes had a significant difference in different chilling-resistant level cotton varieties,within the range of pH4~7,there was a significant difference in the expression level of 50 protein spot... |
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| Keywords: | cotton chilling resistance low-temperature-induced proteins |
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