| 用AFLP片段构建甘蔗祖亲种特异DNA探针 |
| |
| 引用本文： | 庄南生,郑成木,王英,黄东益,高和琼.用AFLP片段构建甘蔗祖亲种特异DNA探针[J].热带作物学报,2004,25(3):18-23. |
| |
| 作者姓名： | 庄南生 郑成木 王英 黄东益 高和琼 |
| |
| 作者单位： | 华南热带农业大学农学院,海南,儋州,571737 |
| |
| 基金项目： | 国家自然科学基金资助项目(30060049) |
| |
| 摘 要： | 应用AFLP标记技术获得了甘蔗祖亲种的AFLP特异片段487条，对部分AFLP特异片段进行斑点杂交与Southern杂交分析，结果有5个可作为甘蔗属特异探针(a44，a59，b60，e42，d19)，2个可作为斑茅特异探针(f08，f16)。利用这些特异探针对参试的30份甘蔗种质进行了血缘测验，能较好地检测出这些种质的基因组构成。其中：崖城73／512、崖城57／25和崖城62／703份种质的血缘与系谱记录不符，均只含甘蔗属血缘而不含斑茅血缘；崖城96／46既含甘蔗属血缘，又含斑茅血缘，因而是甘蔗属与斑茅种杂交的真杂种。
|
| 关 键 词： | AFLP片段 构建技术 甘蔗 亲种 特异DNA探针 |
| 修稿时间： | 2004年3月1日 |
Study on Constructing Specific DNA Probes of Sugarcane Parental Species with AFLP Fragments |
| |
| Zhuang Nansheng,Zheng Chengmu,Wang Ying,Huang Dongyi,Gao Heqiong.Study on Constructing Specific DNA Probes of Sugarcane Parental Species with AFLP Fragments[J].Chinese Journal of Tropical Crops,2004,25(3):18-23. |
| |
| Authors: | Zhuang Nansheng Zheng Chengmu Wang Ying Huang Dongyi Gao Heqiong |
| |
| Abstract: | specific AFLP fragments from sugarcane parental species were selected and obtained using AFLP technique. Among some of these fragments analyzed using dot blotting and Southern hybridization, 5 could be as Saccharum-specific probes (a44, a59, b60, e42, d19),and 2 could be as Eranthus arundinaceus-specific probes (f08, f16). With these specific probes, the genomic components of 30 sugarcane germplasms were tested. The results showed that the genomes of the germplasms were clearly identified with the probes. The results also showed that Yacheng 73/ 512, Yacheng 57/ 25 and Yacheng 62/ 70 did not conform to the historical records of the pedigree because they only contained the genome derived from Saccharum, indicating that E. arundinaceus may not contribute to their genomes. Yacheng 96/46 contained the genomic components of both Saccharum and E. arundinaceus. It was a true hybrid from Saccharum/ E. arundinaceus. |
| |
| Keywords: | sugarcane specific DNA probe AFLP Dot blot Southern hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
