| VB1和干燥处理对小麦幼胚愈伤组织培养的影响 |
| |
| 引用本文： | 郭向云,尹钧,余桂荣,宋丽.VB1和干燥处理对小麦幼胚愈伤组织培养的影响[J].华北农学报,2003,18(4):19-22. |
| |
| 作者姓名： | 郭向云 尹钧 余桂荣 宋丽 |
| |
| 作者单位： | 1. 山西农业大学,山西,太谷,030801
2. 河南农业大学,国家小麦工程技术研究中心,河南,郑州,450002 |
| |
| 基金项目： | 国家转基因植物研究与专业化专项(J2000-B-019);河南省杰出人才创新基金(121000700)资助 |
| |
| 摘 要： | 以14个小麦品种为试验材料，研究不同浓度维生素B1和分化前的干燥处理对小麦幼胚愈伤组织诱导、分化过程的影响。结果表明，小麦幼胚愈伤组织的分化率在不同基因型之间差异显著，其中最高的为郑新992(71．9％)；在愈伤组织诱导、继代以及分化培养的Ms培养基中添加维生素B1(盐酸硫胺素)10mg／L有利于小麦幼胚胚性愈伤组织的发生，同时对促进小麦幼胚愈伤组织的分化也有显著影响；在转入分化培养基前对愈伤组织进行干燥处理(12h)可有效提高其分化频率。
|
| 关 键 词： | VB1 干燥处理 小麦 幼胚 愈伤组织 组织培养 影响因素 |
| 文章编号： | 1000-7091(2003)04-0019-04 |
| 修稿时间： | 2003年1月8日 |
Effects of VB1 and Desiccation on Callus Culture of Wheat Immature Embryos |
| |
| GUO Xiang-yun.Effects of VB1 and Desiccation on Callus Culture of Wheat Immature Embryos[J].Acta Agriculturae Boreali-Sinica,2003,18(4):19-22. |
| |
| Authors: | GUO Xiang-yun |
| |
| Institution: | GUO Xiang-yun~ |
| |
| Abstract: | The effects of VB_(1) and desiccation on callus induction and bud differentiation of immature embryo from 14 wheat genotypes were studied.Result indicated:there was a significant difference between the various genotypes in bud differentiation frequency,and ZhengXin 992 was the highest(71.9%).VB_(1)(10 mg/L) supplemented into the induction?subculture and differentiation media can improved the embryogenic callus development and increased the bud differentiation frequency.12 h desiccation before differentiation culture improved the bud differentiation frequency of calli. |
| |
| Keywords: | Wheat Genotypes VB_(1) Desiccation Callus induction and differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
