| 白木香SRAP—PCR反应体系的建立 |
| |
| 引用本文： | 邹枚伶,夏志强,吉家敏,陈新,王海燕,曾长英,王文泉.白木香SRAP—PCR反应体系的建立[J].广西农业生物科学,2009(1):137-140. |
| |
| 作者姓名： | 邹枚伶 夏志强 吉家敏 陈新 王海燕 曾长英 王文泉 |
| |
| 作者单位： | 中国热带农业科学院热带生物技术研究所; |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(ITBBZD2007-2)资助 |
| |
| 摘 要： | 本实验对白木香SRAP－PCR反应体系的影响因素（模板DNA，Taq酶，Mg^2＋d,NTP，引物）在4个水平上进行优化试验，筛选出各反应因素的最佳水平，建立白木香SRAP－PCR反应的最佳体系（20μL）：模板DNA50ng、引物0．30μmol／L、Mg^2＋ 1．5mmol／L、dNTP0．4mmol／L和购DNA聚合酶1．0U。适用于白木香的SRAP—PCR反应体系迄今未见报道，这一优化系统的建立为今后利用SRAP标记技术对白木香进行基础研究提供了一个标准化的程序。
|
| 关 键 词： | 白木香 SRAP-PCR反应体系 建立 |
Establishment of SRAP-PCR Reaction Conditions in Aquilaria sinensis(Lour.) Spreng |
| |
| Zou Meiling Xia Zhiqiang Ji Jiamin Chen Xin Wang Haiyan Zeng Changying Wang Wenquan Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou.Establishment of SRAP-PCR Reaction Conditions in Aquilaria sinensis(Lour.) Spreng[J].Journal of Guangxi Agricultural and Biological Science,2009(1):137-140. |
| |
| Authors: | Zou Meiling Xia Zhiqiang Ji Jiamin Chen Xin Wang Haiyan Zeng Changying Wang Wenquan Institute of Tropical Bioscience Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou |
| |
| Institution: | Zou Meiling Xia Zhiqiang Ji Jiamin Chen Xin Wang Haiyan Zeng Changying Wang Wenquan Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101 |
| |
| Abstract: | In this paper, the design was used to optimize SRAP-PCR amplification system on Aquilaria sinensis （Lour.） Spreng in four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, dNTP, and primer） respectively. The results showed that the effects of each factor in different levels were found. A most suitable SRAP- PCR system forA quilaria sinensis （Lour.） Spreng was established that is total 20 μL reaction system containing 50 ng DNA template, 0.30 μmol/L primer, 1.5 mmol/L Mg^2＋, 0.4 mmol/L dNTP and 1.0 U Taq DNA polymerase. Until now no SRAP-PCR amplification system base on A quilaria sinensis （Lour.） Spreng have been explored yet. This optimized system for SRAP marker would become one of the protocols for further research in our lab. |
| |
| Keywords: | A quilaria sinensis （Lour ） Spreng SRAP-PCR reaction conditions Establishment |
| 本文献已被 CNKI 维普 等数据库收录！ |
