| 茎瘤芥超氧化物歧化酶活性的研究 |
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| 引用本文： | 何士敏,李吉,唐菊.茎瘤芥超氧化物歧化酶活性的研究[J].安徽农业科学,2011,39(30):18411-18413,18416. |
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| 作者姓名： | 何士敏 李吉 唐菊 |
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| 作者单位： | 长江师范学院生命科学与技术学院,重庆涪陵,408100;长江师范学院生命科学与技术学院,重庆涪陵,408100;长江师范学院生命科学与技术学院,重庆涪陵,408100 |
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| 摘 要： | 目的]揭示茎瘤芥超氧化物歧化酶（SOD）的活性以及pH、底物浓度、温度对SOD活性的影响。方法]以茎瘤芥的瘤茎及叶片为试验材料,用邻苯三酚自氧化法分别测得它们中SOD的活性,以及pH、底物浓度、温度对其活性的影响,从而比较茎瘤芥瘤茎及叶片中SOD的含量,以及是否具有开发利用的价值。结果]茎瘤芥瘤茎SOD的活性在200～300 U/g;茎瘤芥叶片SOD的活性在250～400U/g。说明茎瘤芥叶片SOD活性高于茎瘤芥瘤茎SOD活性。茎瘤芥SOD在pH为8.3～8.6有最大酶活性;最适温度为50℃;最适底物浓度为7 mmol/L左右。结论]茎瘤芥叶片SOD活性高于茎瘤芥瘤茎SOD活性。研究结果为开发茎瘤芥资源提供了参考。
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| 关 键 词： | 超氧化物歧化酶 茎瘤芥 酶活性 邻苯三酚自氧化法 |
Study on the Enzymatic Activity of the Superoxide Dismutase of Brassica juncea Coss.var. tumida Tsen et Lee |
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| HE Shi-min et al.Study on the Enzymatic Activity of the Superoxide Dismutase of Brassica juncea Coss.var. tumida Tsen et Lee[J].Journal of Anhui Agricultural Sciences,2011,39(30):18411-18413,18416. |
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| Authors: | HE Shi-min |
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| Institution: | HE Shi-min et al(Department of Life Science and Technology,Changjiang Teacher College,Fuling,Chongqing 408003) |
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| Abstract: | Objective] The activity of SOD of Brassica juncea Cross.var.tumida Tsen et Lee and the effect of the pH value,the concentration of substrate and temperature on it were explored.Method] The activity of SOD,the effect of the pH value,concentration of substrate and temperature on it of the tumorous stem and leaf of Brassica juncea Cross.var.tumida Tsen et Lee as experimental material were measured with the method of pyrogallicacid oxidize and the value of its developing and using was analyzed.Result] The ac... |
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| Keywords: | Superoxide Dismutase Brassica juncea Coss var tumida Tsen et Lee Enzymatic activity Method of pyrogallicacid oxidize |
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