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| 红霉素发酵培养基的优化研究 | |
| 引用本文： | 左勇,霍丹群,李杨,任永利.红霉素发酵培养基的优化研究[J].安徽农业科学,2011,39(30):18763-18765. |
| 作者姓名： | 左勇 霍丹群 李杨 任永利 |
| 作者单位： | 1. 重庆大学生物工程学院,重庆400030;四川理工学院生物工程学院,四川自贡643000 2. 重庆大学生物工程学院,重庆,400030 3. 四川理工学院生物工程学院,四川自贡,643000 |
| 摘 要： | 目的]对红色糖多孢菌产红霉素的发酵培养基进行优化。方法]采用Design-Expert 7.0软件对发酵培养基的黄豆饼粉、淀粉、糊精和葡萄糖4个组分进行优化,再对硫酸铵、碳酸钙、玉米浆和磷酸二氢钾4个组分进行正交试验优化。结果]最佳培养基配方为黄豆饼粉2.89%、淀粉3.025%、糊精2.04%、葡萄糖1.97%、碳酸钙0.7%、硫酸铵0.1%、玉米浆1.2%、磷酸二氢钾0.04%,优化后发酵产物中红霉素生物效价比优化前提高22.55%。结论]得到了红霉素培养基的优化配方,使红霉素的发酵水平得到较大提高。 |
| 关 键 词： | 红色糖多孢菌 发酵培养基 响应面法 正交试验 |
Optimization of Fermentation Medium for Erythrocin Production |
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| ZUO Yong et al.Optimization of Fermentation Medium for Erythrocin Production[J].Journal of Anhui Agricultural Sciences,2011,39(30):18763-18765. | |
| Authors: | ZUO Yong |
| Institution: | ZUO Yong et al (Bioengineering College of Chongqing University,Chongqing 400030) |
| Abstract: | Objective] To optimize the fermentation medium for producing erythrocin from Saccharopolyspora erythraea.Method] Design-Expert 7.0 was used to design the four factors,including soybean meal,starch,dextrin and glucose.Meanwhile orthogonal test was used to optimize the other four factors of(NH4)2SO4,CaCO3,Corn steep liquor and KH2PO4.Result] The optimal medium was that the concentrations of soybean meal,starch,dextrin,glucose,CaCO3,(NH4)2SO4,corn steep liquor and KH2PO4 were 2.89%,3.025%,2.04%,1.97%,0.7%,0... |
| Keywords: | Saccharopolyspora erythraea Fermentation medium Response surface methodology Orthogonal test |
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