Functional analysis of the venom allergen-like protein gene from pine wood nematode Bursaphelenchus xylophilus using a baculovirus expression system |
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| Institution: | 1. Division of Forest Insect Pest and Diseases, National Institute of Forest Science, Seoul 02455, Republic of Korea;2. Southern Forest Resources Research Center, National Institute of Forest Science, Jinju 52817, Republic of Korea;3. Department of Ophthalmic Optics, Shinhan University, Gyeonggi 11644, Republic of Korea;4. Division of Wood Chemistry & Microbiology, National Institute of Forest Science, Seoul 02455, Republic of Korea |
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| Abstract: | The pine wood nematode, Bursaphelenchus xylophilus, infects pine trees, leading to fatal pine wilt disease. Here, recombinant venom allergen-like protein (VAP) was obtained by expressing Bx-vap-1 in insect cells. Three-year-old Pinus massoniana were inoculated with recombinant VAP, simulating B. xylophilus esophageal gland secretions. Recombinant VAP up-regulated α-pinene synthase gene expression, the trees showed disease symptoms 15 d after inoculation and the xylem pith revealed brown tissue discoloration, indicating that recombinant VAP could damage P. massoniana cells. Recombinant VAP did not, however, lead to cavitation, indicating that the VAP secreted from B. xylophilus acts as a defense response elicitor. |
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| Keywords: | Venom allergen-like protein Insect cell expression α-Pinene synthetase gene |
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