| 美人蕉黄斑驳病毒巢式PCR检测方法的建立 |
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| 引用本文： | 陈细红,杨小龙,廖富荣,高芳銮,沈建国.美人蕉黄斑驳病毒巢式PCR检测方法的建立[J].植物保护,2019,45(4):162-165. |
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| 作者姓名： | 陈细红 杨小龙 廖富荣 高芳銮 沈建国 |
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| 作者单位： | 1. 福建出入境检验检疫局检验检疫技术中心, 福州 350001; 2. 福建农林大学植物病毒研究所, 福州 350002; 3. 厦门出入境检验检疫局检验检疫技术中心, 厦门 361026 |
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| 基金项目： | 国家重点研发计划(2016YFF0203203) |
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| 摘 要： | 本文以我国台湾进境美人蕉病株为材料,根据已报道的美人蕉黄斑驳病毒Canna yellow mottle virus(CaYMV)基因序列设计2对特异性引物(外侧引物1对、内侧引物1对),建立了巢式PCR快速检测CaYMV的方法,并对进境的50份美人蕉样品进行了检测。结果显示,该方法特异性强,且灵敏度高于常规PCR,是常规PCR的1 000倍,表明该方法能够实现对CaYMV的快速、准确、灵敏检测,适用于口岸快速检测CaYMV。
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| 关 键 词： | 美人蕉黄斑驳病毒 巢式PCR 检测 |
| 收稿时间： | 2018/7/3 0:00:00 |
| 修稿时间： | 2018/9/3 0:00:00 |
Development of nested PCR assay for detection of Canna yellow mottle virus |
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| CHEN Xihong,YANG Xiaolong,LIAO Furong,GAO Fangluan,SHEN Jianguo.Development of nested PCR assay for detection of Canna yellow mottle virus[J].Plant Protection,2019,45(4):162-165. |
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| Authors: | CHEN Xihong YANG Xiaolong LIAO Furong GAO Fangluan SHEN Jianguo |
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| Institution: | 1. Inspection and Quarantine Technology Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China; 2. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Inspection and Quarantine Technology Center, Xiamen Entry-ExitInspection and Quarantine Bureau, Xiamen 361026, China |
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| Abstract: | Two pairs of specific primers were designed and synthesized based on the published nucleotide sequence of Canna yellow mottle virus (CaYMV), and then the detection method of nested PCR was established using the total DNA as template, which was extracted from infected canna from Taiwan. The results showed that the nested PCR assay had high specificity and sensitivity. The sensitivity of the nested-PCR assay was higher than that of conventional PCR by 1 000 times. The nested PCR assay established in this study is a rapid, accurate and sensitive detection method for CaYMV, and can be used for rapid detection of CaYMV. |
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| Keywords: | Canna yellow mottle virus nested PCR detection |
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