| 油茶炭疽病拮抗细菌P-14的拮抗物质分析 |
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| 引用本文： | 喻锦秀,何振,李密,邬颖,彭邵锋,钟杰,高必达.油茶炭疽病拮抗细菌P-14的拮抗物质分析[J].林业科学研究,2019,32(1):118-124. |
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| 作者姓名： | 喻锦秀 何振 李密 邬颖 彭邵锋 钟杰 高必达 |
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| 作者单位： | 湖南农业大学, 湖南 长沙 410128;湖南省林业科学院, 湖南 长沙 410004,湖南省林业科学院, 湖南 长沙 410004,湖南省林业科学院, 湖南 长沙 410004,湖南林科达农林技术服务有限公司, 湖南 长沙 410004,湖南省林业科学院, 湖南 长沙 410004;国家油茶工程技术研究中心, 湖南 长沙 410004,湖南农业大学, 湖南 长沙 410128,湖南农业大学, 湖南 长沙 410128 |
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| 基金项目： | 湖南省林业科技计划项目“油茶主要病害、钻蛀性害虫生物防治技术研究”（YC2016001）；湖南省科技重大专项“油茶良种繁育与生态高效培育关键技术研究与示范”（2013FJ1006） |
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| 摘 要： | 目的]探索解淀粉芽孢杆菌P-14对油茶炭疽病病原菌的拮抗物质,以期为油茶炭疽病的生物农药开发利用提供理论依据。方法]利用酸沉淀法和高效液相色谱法分离解淀粉芽孢杆菌P-14发酵液中的拮抗物质,通过顶空固相微萃取法和气相色谱-质谱法对解淀粉芽孢杆菌P-14的挥发性物质进行了分离鉴定。结果]分离后的组分a对油茶炭疽病的病原菌具有良好的抑制作用,其主要成分为C15 bacillomycin D(杆菌霉素D)。此外,共分离得到35种挥发性化合物,其相对抑菌率为(26.19±3.82)%,其中包含苯类物质(benzenes)10种、烷基类物质(alkyls)8种、醇类物质(alcohols)2种、酮类物质(ketones)11种、醛类物质(aldehydes)3种和1种酯类物质(esters)。结论]解淀粉芽孢杆菌P-14能产生对油茶炭疽病的病原菌具有明显的拮抗作用的物质,并确定了其拮抗物质种类,为油茶炭疽病的生物农药开发提供了可靠的研究内容。
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| 关 键 词： | 油茶炭疽病 解淀粉芽孢杆菌 拮抗物质 脂肽类抗生素 挥发性物质 |
| 收稿时间： | 2017/11/9 0:00:00 |
| 修稿时间： | 2018/3/28 0:00:00 |
Analysis of Metabolic Antagonists Against Colletotrichum camellia by Antagonistic Bacteria P-14 |
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| YU Jin-xiu,HE Zhen,LI Mi,WU Ying,PENG Shao-feng,ZHONG Jie and GAO Bi-da.Analysis of Metabolic Antagonists Against Colletotrichum camellia by Antagonistic Bacteria P-14[J].Forest Research,2019,32(1):118-124. |
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| Authors: | YU Jin-xiu HE Zhen LI Mi WU Ying PENG Shao-feng ZHONG Jie and GAO Bi-da |
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| Institution: | Hunan Agricultural University, Changsha 410128, Hunan, China;Hunan Academy of Forestry, Changsha 410004, Hunan, China,Hunan Academy of Forestry, Changsha 410004, Hunan, China,Hunan Academy of Forestry, Changsha 410004, Hunan, China,Hunan Linkeda Agriculture and Forestry Technology Service Co., Ltd, Changsha 410004, Hunan, China,Hunan Academy of Forestry, Changsha 410004, Hunan, China;National Oil-tea Camellia Engineering & Technology Research Center, Changsha 410004, Hunan, China,Hunan Agricultural University, Changsha 410128, Hunan, China and Hunan Agricultural University, Changsha 410128, Hunan, China |
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| Abstract: | Objective] The purpose of this study is to explore the metabolic antagonists against Colletotrichum camellia produced by antagonistic bacteria P-14, in order to provide a theoretical basis for the development and utilization of biopesticides of Camellia oleifera. Method] The acid precipitation and high-performance liquid chromatography was used to identify the lipopeptide antibiotics produced by strain P-14, and the volatile compounds were analyzed by gas chromatography-mass spectrometry. Result] The component a containing C15 bacillomycin D showed the strongest inhibitory effect against Colletotrichum camellia. In addition, a total of 35 volatile compounds were separated, the relative bacteriostatic rate was (26.19±3.82)%, including 10 benzenes, 8 alkyls, 2 alcohols, 11 ketones, 3 aldehydes, and 1esters. Conclusion] Bacillus amyloliquefaciens P-14 could produce metabolic antagonists against Colletotrichum camellia, and the metabolic antagonists has been identified, which can provide with reliable research data for the development of biopesticides against C. oleifera. |
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| Keywords: | Colletotrichum camellia Bacillus amyloliquefaciens metabolic antagonists lipopeptide volatile organic compounds |
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