| 黑龙江省稻田野慈姑对丙嗪嘧磺隆抗性相关研究 |
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| 引用本文： | 吕晓曦,赵长山,李 庚,王春雨,吴 桐.黑龙江省稻田野慈姑对丙嗪嘧磺隆抗性相关研究[J].植物保护,2019,45(2):174-177. |
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| 作者姓名： | 吕晓曦 赵长山 李 庚 王春雨 吴 桐 |
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| 作者单位： | 东北农业大学农学院, 哈尔滨 150030 |
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| 基金项目： | 黑龙江省科技厅科技攻关课题（GC12B103） |
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| 摘 要： | 通过整株盆栽法研究黑龙江省佳木斯市汤原县(种群R1)、856农场(种群R2)、密山市(种群R3)3个水稻田野慈姑种群对丙嗪嘧磺隆的抗性水平,采用分子生物学技术分析3个野慈姑种群在靶标酶基因上的差异,确定3个野慈姑种群对丙嗪嘧磺隆和苄嘧磺隆是否存在交互抗性。结果显示,黑龙江R1、R2、R3种群抗性指数(RI)分别为11.92、22.68、35.99。与敏感的七台河种群S相比,R1、R2、R3的ALS基因均在Pro_(197)位发生不同突变。R1种群为Thr_(197)取代了Pro_(197);R2、R3种群为Ser_(197)取代了Pro_(197),ALS基因的突变是其产生抗性的主要原因。3个野慈姑种群对丙嗪嘧磺隆和苄嘧磺隆存在交互抗性。
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| 关 键 词： | 野慈姑 丙嗪嘧磺隆 抗性 |
| 收稿时间： | 2018/4/30 0:00:00 |
| 修稿时间： | 2018/7/5 0:00:00 |
Resistance of Sagittaria trifolia L. populations in rice fields in Heilongjiang Province to propyrisulfuron |
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| LV Xiaoxi,ZHAO Changshan,LI Geng,WANG Chunyu,WU Tong.Resistance of Sagittaria trifolia L. populations in rice fields in Heilongjiang Province to propyrisulfuron[J].Plant Protection,2019,45(2):174-177. |
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| Authors: | LV Xiaoxi ZHAO Changshan LI Geng WANG Chunyu WU Tong |
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| Institution: | School of Agriculture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | The whole-plant bioassays were used to determine the resistance level of Sagittaria trifolia populations from rice fields of Tangyuan (R1), 856 farm (R2) and Mishan in Heilongjiang Province to propyrisulfuron. The target genes of the resistance of the three S. trifolia populations were analyzed and cross-resistances between the three S. trifolia populations to propyrisulfuron and bensulfuron-methyl were determined by molecular biology techniques. The results showed that the resistance indexes (RIs) were 11.92, 22.68 and 35.99 for R1, R2 and R3, respectively. Compared with the susceptible population, the acetolactate synthase (ALS) genes of R1, R2, and R3 all had different mutations at Pro197. The amino acid substitution for R1 population was Pro-197-Thr, and that for R2 and R3 populations was Pro-197-Ser. The results showed that the mutation of ALS gene is the main reason for S. trifolia resistance. Three S. trifolia populations developed cross-resistance to bensulfuron-methyl and propyrisulfuron. |
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| Keywords: | Sagittaria trifolia propyrisulfuron resistance |
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