| 响应面法优化燕窝不溶性蛋白酶解工艺的研究 |
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| 引用本文： | 郑心怡,李致瑜,曾红亮,郑宝东,张 怡.响应面法优化燕窝不溶性蛋白酶解工艺的研究[J].中国农学通报,2019,35(6):151-157. |
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| 作者姓名： | 郑心怡 李致瑜 曾红亮 郑宝东 张 怡 |
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| 作者单位： | 1.福建农林大学食品科学学院;2.福建农林大学中国-爱尔兰国际合作食品物质学与结构设计研究中心 |
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| 基金项目： | 福建农林大学国际科技合作与交流资助项目“中爱国际合作食品物质学与结构设计研究中心”(KXGH17001)；福建省科技创新领军人才 (KRC16002A)；福建农林大学科技创新专项基金立项项目(CXZX2017018)；福建农林大学科技创新专项基金立项项目(CXZX2017414)。 |
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| 摘 要： | 研究旨在应用酶解技术将燕窝中不溶性蛋白转换为水溶性多肽，使分子量降低，有利于人体消化吸收，以期为燕窝新产品的开发奠定基础。以水解度和肽得率为指标，筛选出碱性蛋白酶对燕窝中不溶性蛋白进行酶解。通过单因素实验研究酶解时间、酶加量、底物浓度对燕窝多肽得率的影响，并利用响应曲面法对燕窝不溶性蛋白酶解工艺进行优化。结果表明：酶解的最佳工艺为：底物浓度为2.5%，酶加量为4%，酶解时间为3.6h，得到的肽得率为33.59%。通过实验对该模型进行验证：得到肽得率为33.38%，相对误差为0.56%，说明该模型能较好地反映出燕窝不溶性蛋白酶解情况。此外，通过凝胶电泳实验证明燕窝不溶性蛋白通过碱性蛋白酶酶解，分子量有效降低。该实验获得了燕窝多肽，为后续产品进一步深入开发和应用提供一定理论基础。
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| 关 键 词： | 包被复合微量元素 包被复合微量元素 黄羽肉鸡 生长性能 粪便微量元素含量 小肠黏膜形态 |
| 收稿时间： | 2018/9/3 0:00:00 |
| 修稿时间： | 2018/10/18 0:00:00 |
The Response Surface Methodology Optimizes Enzymatic Hydrolysis Process of Insoluble Protein in Edible Bird’s Nest |
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| Abstract: | The research aims to use the enzymolysis technology to convert the insoluble protein from edible bird’s nest into water-soluble peptide, which could facilitate the digestion and absorption by the human body, and provide a foundation for the development of new products of edible bird’s nest. Using the hydrolyzing degree and the yield of peptide as index, alkaline protease was selected to hydrolyze the insoluble protein from edible bird’s nest. The effects of enzymolysis time, enzyme amount and substrate concentration on the yield of edible bird’s nest peptide were studied with single factor experiment. The response surface analysis was used to optimize the enzymatic conditions of the insoluble protein from edible bird’s nest. The optimal condition of enzymatic hydrolysis was: substrate concentration of 2.5%, the enzyme amount of 4%, enzymolysis time of 3.6 h, and the yield of peptide of 33.59%. The model was verified by the experiment: the yield of the peptide was 33.38%, and the relative error was 0.56%, which indicated that the model could well reflect the enzymatic hydrolysis of the insoluble protein from edible bird’s nest. In addition, gel electrophoresis experiments showed that the molecular mass of the insoluble protein was effectively reduced by using alkaline protease to hydrolyze the insoluble protein. |
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| Keywords: | bird"s nest peptide response surface SDS-PAGE |
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