| 伊氏杀线真菌与苏云金芽孢杆菌对松材线虫的联合毒力研究 |
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| 引用本文： | 李恩杰,李娜,王青华,张永安,王玉珠,曲良建.伊氏杀线真菌与苏云金芽孢杆菌对松材线虫的联合毒力研究[J].林业科学研究,2019,32(1):106-111. |
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| 作者姓名： | 李恩杰 李娜 王青华 张永安 王玉珠 曲良建 |
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| 作者单位： | 中国林业科学研究院华北林业实验中心, 北京 102300;中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护重点实验室, 北京 100091,内蒙古农业大学林学院, 内蒙古 呼和浩特 010019,中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护重点实验室, 北京 100091,中国林业科学研究院华北林业实验中心, 北京 102300;中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护重点实验室, 北京 100091,中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护重点实验室, 北京 100091,中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护重点实验室, 北京 100091 |
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| 基金项目： | 中国林科院森环森保所中央级公益性科研院所基本科研业务费专项资金项目：伊氏真菌对松材线虫致病相关基因的研究（CAFRIFEEP201403）、林业公益性行业科研专项：重大森林害虫持续防控关键技术与体系研究 （201504302） |
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| 摘 要： | 目的]探究伊氏杀线真菌与苏云金芽孢杆菌(Bacillus thuringiensis,Bt)对松材线虫的联合毒力。方法]使用Esteya vermicola孢子悬浮液与Bt发酵液处理松材线虫,通过线虫的形态变化、死亡率及死亡速度3个方面测定E.vermicola与Bt联合对松材线虫的影响。结果]经过E.vermicola孢子悬浮液处理过的线虫会出现内容物渗漏,体腔收缩、弯曲、断裂的现象;高浓度的E.vermicola孢子悬浮液与Bt发酵液联合处理松材线虫可以明显地提高线虫的死亡率,最高可以达到100%;联合处理的线虫死亡速度较快,随着处理时间的延长,死亡率会一直呈上升的趋势。结论]应用这两种生防微生物在合适的浓度下混配能够在较短时间内提高松材线虫的死亡率。
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| 关 键 词： | Esteya vermicola孢子悬浮液 Bt发酵液 松材线虫 死亡率 |
| 收稿时间： | 2017/11/3 0:00:00 |
| 修稿时间： | 2018/3/24 0:00:00 |
Study on Joint Toxicities of Esteya vermicola and Bacillus thuringiensis to Pine Wood Nematode (Bursaphelenchus xylophilus) |
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| LI En-jie,LI N,WANG Qing-hu,ZHANG Yong-an,WANG Yu-zhu and QU Liang-jian.Study on Joint Toxicities of Esteya vermicola and Bacillus thuringiensis to Pine Wood Nematode (Bursaphelenchus xylophilus)[J].Forest Research,2019,32(1):106-111. |
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| Authors: | LI En-jie LI N WANG Qing-hu ZHANG Yong-an WANG Yu-zhu and QU Liang-jian |
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| Institution: | Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China;Research Institute ofForest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, National Forestry andGrassland Administration, Beijing 100091, China,College of Forestry, Inner Mongolia Agricultural University, Huhehot 010019, Inner Mongolia, China,Research Institute ofForest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, National Forestry andGrassland Administration, Beijing 100091, China,Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China;Research Institute ofForest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, National Forestry andGrassland Administration, Beijing 100091, China,Research Institute ofForest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, National Forestry andGrassland Administration, Beijing 100091, China and Research Institute ofForest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, National Forestry andGrassland Administration, Beijing 100091, China |
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| Abstract: | Objective] To explore the toxicities of Esteya vermicola in combination with Bacillus thuringiensis (Bt) against Bursaphelenchus xylophilus. Method] The joint toxicities of E. vermicola and Bt against pine wood nematodes was measured by the morphological changes, mortality and death rate of nematodes treated with E. vermicola spore suspension and Bt fermentation supernatant. Result] The results showed that the nematodes treated by E. vermicola spore suspension could cause leakage of body contents and shrinkage, bending and fracture of body cavity. The combination of high concentration of E. vermicola spore suspension and Bt fermentation solution could significantly increase the mortality of nematodes, with a maximum of 100%. The combined treatment of pine wood nematodes died faster, and the mortality rate would continue to rise with the extension of treatment time. Conclusion] Application of these two bio-control agents at the right concentrations can increase the mortality of pine wood nematodes in a relatively short time. |
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| Keywords: | Esteya vermicola spore suspension Bacillus thuringiensis fermentation supernatant pine wood nematode mortality |
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