| 红芽芋超低温疗法脱毒苗基因组DNA甲基化的MSAP分析 |
| |
| 引用本文： | 尹明华,廖 玉,万志庭.红芽芋超低温疗法脱毒苗基因组DNA甲基化的MSAP分析[J].西北农业学报,2019,28(1):132-138. |
| |
| 作者姓名： | 尹明华 廖 玉 万志庭 |
| |
| 作者单位： | （上饶师范学院 生命科学学院，江西上饶 334001） |
| |
| 基金项目： | 国家自然科学基金（31860084）；2016年度江西省教育厅科学技术研究项目（GJJ161053）。 |
| |
| 摘 要： | 对红芽芋超低温疗法脱毒苗的基因组DNA甲基化进行MSAP分析。结果表明,红芽芋超低温疗法脱毒苗总甲基化率提高10.27%,全甲基化率提高12.98%,但半甲基化率下降2.71%。红芽芋超低温疗法脱毒苗去甲基化模式和甲基化模式并存,但去甲基化模式高于甲基化模式,说明较多基因被激活。
|
| 关 键 词： | 红芽芋 超低温疗法脱毒苗 DNA甲基化 MSAP分析 |
MSAP Analysis of Genomic DNA Methylation in Virus-free Plantlets by Cryotherapy of Red Bud Taro |
| |
| YIN Minghu,LIAO Yu and WAN Zhiting.MSAP Analysis of Genomic DNA Methylation in Virus-free Plantlets by Cryotherapy of Red Bud Taro[J].Acta Agriculturae Boreali-occidentalis Sinica,2019,28(1):132-138. |
| |
| Authors: | YIN Minghu LIAO Yu and WAN Zhiting |
| |
| Abstract: | In this paper,MSAP was used to analyze genomic DNA methylation in virus-free plantlets cryotherapy by red bud taro was used.The results showed that the total methylation rate increased by 10.27%,the full-methylation rate increased by 12.98%,but the hemi-methylation rate decreased by 2.71%. The demethylation and methylation modes in virus-free plantlets by cryotherapy of red bud taro were coexisted,but the demethylation mode was higher than the methylation mode,it indicated that more gene expression was activated. The results of this study can provide a theoretical basis for germplasm conservation and large-scale production of virus-free plantlets by cryotherapy of red bud taro. |
| |
| Keywords: | Red bud taro Virus-free plantlets by cryotherapy DNA methylation MSAP analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
