| 栉孔扇贝血淋巴中酚氧化酶和髓过氧物酶活性↑（*） |
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| 引用本文： | 孙虎山,李光友.栉孔扇贝血淋巴中酚氧化酶和髓过氧物酶活性↑（*）[J].中国水产科学,1999,6(2):9-13. |
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| 作者姓名： | 孙虎山 李光友 |
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| 作者单位： | 1. 烟台师范学院,烟台,264025
2. 国家海洋局第一海洋研究所,青岛,266003 |
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| 摘 要： | 用电镜细胞化学和分光光度技术对栉孔扇贝血淋巴中的氧化酶（ＰＯ）和髓过氧物酶（ＭＰＯ）进行了研究。结果表明：细胞质中直径分别为３００～４３０ｎｍ和１３０～２００ｎｍ的大、小２种颗粒呈ＰＯ强阳性，次级溶酶体和小颗粒呈ＭＰＯ强阳性。生化血清中存在ＰＯ和ＭＰＯ活性，血细胞仅有ＭＰＯ活性。以ＴＭＢ为底物测得血清中不依赖卤素的ＭＰＯ活力为１０７．２Ｕ，以二乙醇胺为底物测得血清中依赖卤素的ＴＭＢ为底物测得血清中
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| 关 键 词： | 栉孔扇贝 酚氧化酶 髓过氧物酶 酶活性 血淋巴 |
| 修稿时间： | 2010/4/8 0:00:00 |
Phenoloxidase and myeloperoxidase activity in the haemocytes and serum of Chlamys farreri |
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| Sun Hushan,Li Guangyou.Phenoloxidase and myeloperoxidase activity in the haemocytes and serum of Chlamys farreri[J].Journal of Fishery Sciences of China,1999,6(2):9-13. |
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| Authors: | Sun Hushan Li Guangyou |
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| Abstract: | The phenoloxidase (PO) and myeloperoxidase(MPO)in the haemocytes and haemolymph of the Chlamys frrreri were studied with the method of cytochemical localisation using electron-microscopy and specreophotometer.The results show that 2 kinds of granules,300430 nm and 130200 nm in diameter,in cytoplasm are strongly positive to PO. The secondary lysosomes and the small granules are strongly positive to MPO. The results of biochemical test show that there are PO and MPO in haemolymph and there is only MPO in haemocytes. PO is present in an inactive form (proPO) in haemocyres. The enzyme activities of MPO in haemocytes is 3.5 fold as much as those in the haemolymph. Halide-independent MPO in haemolymph is 107.2 U using TMB as the substrate, and halide-dependent MPO in haemolymph is 53.6 U using diethanolamine as the substrate.The optimum pH of PO and MPO in haemolymph are 6.0 and 6.5 and the optimum temperatures of PO and MPO in haemolymph are 45 and 35. |
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| Keywords: | Chlamys farreri phenoloxidase myeloperoxidase enzymatic activity haemolymph |
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