RFLPs of the rRNA genes in the genusPhaseolus |
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| Authors: | Maren Jacob Dorothea Zink Walter Nagl |
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| Institution: | 1. Department of Biology, and The Biotechnology Program, University of Kaiserslautern, 67653, Kaiserslautern, Germany
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| Abstract: | Restriction fragment length polymorphisms of the rRNA genes were investigated among 20 genotypes ofP. vulgaris, P. coccineus, P. polyanthus, P. microcarpus, P. glabellus, P. acutifolius, P. maculatus, P. oligospermus andP. lunatus. Detection of the polymorphisms was performed nonradioactively with a digoxigenin-labeled rDNA probe. RFLP-based phylogenetic trees for the rDNA of the species studied were computed with several distance matrix and parsimony methods. The estimated molecular relationships within the genusPhaseolus coincide, on principle, with the classical taxonomical investigations. Hence, RFLP analyses of the rRDA have been proven useful for systematic studies in the genusPhaseolus. |
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