| 日本三角涡虫全能干细胞的分离培养与鉴定 |
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| 引用本文： | 王秀革,刘旭东,于倩,黄金明,王长法,袁金铎.日本三角涡虫全能干细胞的分离培养与鉴定[J].山东农业科学,2013,45(2):34-37. |
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| 作者姓名： | 王秀革 刘旭东 于倩 黄金明 王长法 袁金铎 |
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| 作者单位： | 1. 山东师范大学生命科学学院,山东济南,250014
2. 山东省农业科学院奶牛研究中心,山东济南,250131 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金项目(BS2010SW031) |
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| 摘 要： | 涡虫具有极强的再生能力,并且认为涡虫体内存在一类具有增殖分化潜能被称为neoblasts的全能干细胞。这种细胞在涡虫体内可发生迁移、增殖和分化,对其个体组织器官损伤的修复具有重要作用。本实验中,三角涡虫经过饥饿处理后切取特定的组织块,然后再经过处理使组织块中的细胞分离出来。分离的细胞继续培养并通过碱性磷酸酶检测最终鉴定为neoblasts干细胞。
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| 关 键 词： | 三角涡虫 全能干细胞 分离 培养 鉴定 |
Isolation,Culture and Identification of Neoblasts in Dugesia japonica Ichikawa et Kawakatsu |
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| Wang XiuGe
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Liu XuDong
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Yu Qian
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Huang JinMing
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Wang ChangFa
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Yuan JinDuo.Isolation,Culture and Identification of Neoblasts in Dugesia japonica Ichikawa et Kawakatsu[J].Shandong Agricultural Sciences,2013,45(2):34-37. |
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| Authors: | Wang XiuGe Liu XuDong Yu Qian Huang JinMing Wang ChangFa Yuan JinDuo |
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| Institution: | 1*(1.College of Life Science,Shandong Normal University,Jinan 250014,China; 2.Research Center of Dairy Cattle,Shandong Academy of Agricultural Sciences,Jinan 250131,China) |
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| Abstract: | The planarian has strong ability to regenerate,and it is regarded that there are a class of totipotent stem cells called neoblasts,which have proliferative and differentiated potence.This cell migration,proliferation and differentiation in the planarian body play a significant role in repairing damage tissues and organs.In this study,specific tissues were cut from the planarian after starvation,and processed to isolate cells.The isolated cells were cultured and finally identified as neoblasts stem cells by alkaline phosphatase detection. |
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| Keywords: | Planarian Neoblasts Isolation Culture Identification |
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