| 蛇蛔虫ITS及5.8S rDNA的克隆及进化分析 |
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| 引用本文： | 郑姣妹,李芬,陈文承,刘毅.蛇蛔虫ITS及5.8S rDNA的克隆及进化分析[J].中国兽医学报,2012,32(5):688-691. |
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| 作者姓名： | 郑姣妹 李芬 陈文承 刘毅 |
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| 作者单位： | 1. 湖南农业大学动物医学院,湖南长沙410128／湖南省长沙市畜禽水产品质量检测中心,湖南长沙410013
2. 湖南农业大学动物医学院,湖南长沙,410128 |
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| 基金项目： | 国家自然科学基金资助项目(30771616);长沙科技计划一般资助项目(K0902144-21) |
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| 摘 要： | 利用聚合酶链反应（PCR）扩增蛇蛔虫rDNA的ITS-1、5.8S及ITS-2片段,将PCR扩增出的片段纯化后克隆至pGEM-T Easy载体,重组质粒通过菌落PCR鉴定后,对阳性菌落进行序列测定并进行序列分析。结果显示,所获得的蛇蛔虫ITS及5.8SrDNA序列总长为846bp,包含部分的18S、28S及全部的ITS-1、5.8S及ITS-2序列。本研究系国际上首次报道蛇蛔虫的ITS序列,从而为蛇蛔虫的分类鉴定以及进一步的分子流行病学调查奠定了基础。
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| 关 键 词： | 蛇蛔虫 内转录间隔区(ITS) 序列分析 种系发育关系 |
Cloning and phylogenetic analysis of the ITS and 5.8S rDNA of ascarid nematodes from Zaocys dhumnades |
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| ZHENG Jiao-mei,.,LI Fen,CHEN Wen-cheng,LIU Yi.Cloning and phylogenetic analysis of the ITS and 5.8S rDNA of ascarid nematodes from Zaocys dhumnades[J].Chinese Journal of Veterinary Science,2012,32(5):688-691. |
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| Authors: | ZHENG Jiao-mei LI Fen CHEN Wen-cheng LIU Yi |
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| Institution: | 1*(1.Center for Quality Quarantine of Livestock,Poultry and Aquaculture Products,Changsha 410006,China;2.College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | The ITS sequences were amplified from each ascarid sample and the amplicons were cloned into pGEM-T Easy vector,respectively.The inserts were successfully sequenced,and the length of ITS in all samples were 846 bp.Sequence analysis revealed that the isolates contained the seguence of complete ITS-1,5.8 S and ITS-2 rDNA.It was the first time report of the complete sequence of ITS and 5.8S rDNA of ascarid nematodes from Zaocys dhumnades.The results of this study lay down the foundation for further study on molecular epidemiology and diagnostics of ascarid nematodes from snakes. |
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| Keywords: | Zaocys dhumnades ascarid nematode ITS sequence analysis relationship |
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