| 受精液中肝素和咖啡因的组合对牛卵母细胞体外受精的影响 |
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| 引用本文： | 文国艺,卢克焕.受精液中肝素和咖啡因的组合对牛卵母细胞体外受精的影响[J].广西农业生物科学,1994(1). |
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| 作者姓名： | 文国艺 卢克焕 |
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| 作者单位： | 广西农业大学动物繁殖研究室 |
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| 摘 要： | 采用２种肝素浓度（１０，３０ｍｇ／Ｌ）与３种咖啡因浓度（２．５，５，１０ｍｍｏｌ／Ｌ）的交叉组合，以３０ｍｇ／Ｌ肝素的受精液为对照，探讨肝素和咖啡因对牛卵母细胞体外受精的影响，并确定受精后将卵母细胞移至单层细胞的合适时间。结果表明，当卵母细胞在受精后５ｈ移至单层细胞时，１０ｍｇ／Ｌ肝素加５ｍｍｏｌ／Ｌ咖啡因或３０ｍｇ／Ｌ肝素加２．５ｍｍｏｌ／Ｌ咖啡因的体外受精效果最好，二者的囊胚率和囊胚孵化率分别为５６．９％±７．０，８１．１％±１２．７及５６．９％±７．０，７８．９％±９．８。
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| 关 键 词： | 牛卵母细胞 肝素 咖啡因 体外受精 囊胚率 |
EFFECT OF HEPARIN AND CAFFEINE ON THE IN VITRO FERTILIZATION OF BOVINE OOCYTES |
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| Wen Guoyi, Lu Kehuan.EFFECT OF HEPARIN AND CAFFEINE ON THE IN VITRO FERTILIZATION OF BOVINE OOCYTES[J].Journal of Guangxi Agricultural and Biological Science,1994(1). |
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| Authors: | Wen Guoyi Lu Kehuan |
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| Abstract: | A 2 (heparin, 10, 30 mg/L) X 3 (caffeine, 2. 5, 5, 10 mmol/L) factorial experiment was designed to determined the effect of different concentration of heparin and caffeine in fertilization medium(FM)on cleavage rate of bovine oocytes and their subsequent development to blastocyst(BL)stage and the optimal transfer time of oocytes to granulosa cell monolayers after insemination. The results showed that the optimal concen tration of heparin and caffeine in FM was 10 mg/L heparin+5 mmol/L caffeine or 30 mg/ L heparin +2. 5 mmol/L caffeine when oocytes were transferred to granulosa cell monolayers at 5 h after insemination.The BL rate and BL hatching rate for these two combinations of heparin and caffeine were 56. 9%, 81.1 %,and 56.9,%, 78. 9%, respectively. |
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| Keywords: | bovine oocyte heparin caffeine in vitro fertilization blastocycst rate |
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