| 茶树冷胁迫诱导抗寒基因CBF的克隆与表达分析 |
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| 引用本文： | 陈暄,房婉萍,邹中伟,王玉花,成浩,黎星辉.茶树冷胁迫诱导抗寒基因CBF的克隆与表达分析[J].茶叶科学,2009,29(1). |
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| 作者姓名： | 陈暄 房婉萍 邹中伟 王玉花 成浩 黎星辉 |
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| 作者单位： | 1. 中国农业科学院茶叶研究所,浙江,杭州,310008;南京农业大学茶叶科学研究所,江苏,南京,210095
2. 南京农业大学茶叶科学研究所,江苏,南京,210095 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金，江苏省科技攻关项目，国家自然科学基金，南京农业大学青年科技创新基金，江苏省农业三项工程项目，中国博士后科学基金，常州市科技攻关计划 |
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| 摘 要： |
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| 关 键 词： | 茶树 CBF基因 克隆 表达分析 |
Cloning and Expression Analysis of CBF Gene in Cold Induced Tea Plant [Camellia Sinensis (L.)O.Kuntze] |
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| CHEN Xuan,FANG Wan-ping,ZOU Zhong-wei,WANG Yu-hua,CHENG Hao,LI Xing-hui.Cloning and Expression Analysis of CBF Gene in Cold Induced Tea Plant [Camellia Sinensis (L.)O.Kuntze][J].Journal of Tea Science,2009,29(1). |
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| Authors: | CHEN Xuan FANG Wan-ping ZOU Zhong-wei WANG Yu-hua CHENG Hao LI Xing-hui |
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| Institution: | 1. Tea Research Institute;Chinese Academy of Agricultural Sciences;Hangzhou 310008;China;2. Tea Research Institute;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | The cDNA-AFLP was applied to identify genes expressed differentially in cold acclimatized tea plant. A cDNA fragment, TDF11 encoding a CBF (C-repeat binding factor) protein was isolated and identified. The cDNA fragment had 94%、84%、81% homology with CBF genes from Brassica napus, Arabidopsis thaliana, Nicotiana tabacum by BLAST on NCBI. The complete cDNA sequence was cloned by RACE (rapid amplification of cDNA ends), named Camellia sinensis CBF gene. Its full cDNA sequence was 981bp (GenBank accession number EU857638) and contained an open reading frame encoding a polypeptide of 275 amino acid residues. Analysis of the nucleotide sequence and deduced amino acid sequence showed 74%、57%、57% homology with CBF genes from Brassica napus, Nicotiana tabacum, Arabidopsis thaliana. To identify differential expression of CBF gene, RT-PCR experiments were performed in six samples including leaves cold-induced for different treatments. The results showed that tea CBF gene expressed after cold induced for 4 hours and up to a maximum when cold induced for 8 h, and then decreased. It hinted the CBF gene might play a key role during the development of cold resistant molecular mechanism in tea plant. |
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| Keywords: | tea plant (Camellia sinensis) CBF gene clone expression analysis |
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