| 剑麻斑马纹病菌5个多聚半乳糖醛酸酶基因的克隆与序列分析 |
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| 引用本文： | 吴伟怀,梁艳琼,郑金龙,习金根,郑肖兰,李 锐,刘巧莲,张驰成,贺春萍,易克贤.剑麻斑马纹病菌5个多聚半乳糖醛酸酶基因的克隆与序列分析[J].热带作物学报,2015,36(12):2204-2209. |
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| 作者姓名： | 吴伟怀 梁艳琼 郑金龙 习金根 郑肖兰 李 锐 刘巧莲 张驰成 贺春萍 易克贤 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院热带生物技术研究所;海南大学环境与植物保护学院;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室 |
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| 基金项目： | 海南省自然科学基金项目(No. 314105)；国家麻类产业技术体系建设专项资金（No. CARS-19）；中国热带农业科学院环植所自主选题项目(No. 2013hzsJY04)；中央级公益性科研院所基本科研业务费专项(No. 2014hzs1J012)。 |
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| 摘 要： | 以寄生疫霉菌多聚半乳糖醛酸酶pppg1～pppg5等5个基因cDNA序列为参考，设计基因编码区特异性引物。利用5对引物分别对剑麻斑马纹病菌进行分子检测以及基因同源克隆。通过此方法首次从剑麻斑马纹病菌中获得了5个多聚半乳糖醛酸酶基因，并分别命名为Szpg1～Szpg5。检测结果表明，Szpg1～Szpg5基因普遍存在于被检测剑麻斑马纹病菌中。序列分析结果表明，Szpg1～Szpg5基因与pppg1～pppg5对应基因之间存在核苷酸序列差异，由此导致个别氨基酸的差异，甚至提前终止。由此推测，Szpg1～Szpg5基因与pppg1～pppg5在功能上可能存在着一定的差异。
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| 关 键 词： | 剑麻斑马纹病 多聚半乳糖醛酸酶 基因克隆 |
Cloning and Sequence Analysis of Five Polygalacturonase Genes of Phytophora nicotianae in Sisal |
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| WU Weihuai,LIANG Yanqiong,ZHENG Jinlong,XI Jingen,ZHENG Xiaolan,LI Rui,LIU Qiaolian,ZHANG Chicheng,HE Chunping and YI Kexian.Cloning and Sequence Analysis of Five Polygalacturonase Genes of Phytophora nicotianae in Sisal[J].Chinese Journal of Tropical Crops,2015,36(12):2204-2209. |
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| Authors: | WU Weihuai LIANG Yanqiong ZHENG Jinlong XI Jingen ZHENG Xiaolan LI Rui LIU Qiaolian ZHANG Chicheng HE Chunping and YI Kexian |
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| Institution: | Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Institute of Tropical Biosciences and Biotechnology, CATAS;College of Environment and Plant Protection, Hainan University;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS / Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests |
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| Abstract: | Sisal zebra disease caused by Phytophora nicotianae var. parasitica is a kind of main diseases which cause serious damage to the sisal. In this study, five specific primer pairs were designed in coding region based on the reference sequence of pppg1 to pppg5 gene from Phytophora parasitica. Five specific primer pairs were used for the molecular detection and gene cloning from sisal zebra pathgoen DNA. This is the first time from sisal zebra isolating five polygalacturonase genes through this method, and respectively designated as Szpg1 to Szpg5. The detecting results showed that Szpg1 to Szpg5 genes were widely distributed in the sisal zebra isolates. Sequence analysis results showed that there were sequence differences between Szpg1 to Szpg5 and pppg1 to pppg5, respectively, leading to some amino acid differences, and even early termination. Thus it is speculated that Szpg1 to Szpg5 genes may exist a certain differences in function. This research would lay a solid foundation for the further study of sisal zebra germs polygalaturonase role in the pathogenic process. |
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| Keywords: | Sisal zebra disease Polygalaturonase Cloning |
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