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| 鹿流行性出血病病毒VP7基因的克隆及原核表达 | |
| 引用本文： | 杨俊兴,花群义,陈焕春,陈兵,吕建强,曹琛福,阮周曦,张彩红.鹿流行性出血病病毒VP7基因的克隆及原核表达[J].动物医学进展,2009,30(8):5-8. |
| 作者姓名： | 杨俊兴 花群义 陈焕春 陈兵 吕建强 曹琛福 阮周曦 张彩红 |
| 作者单位： | 1. 深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳,518001;华中农业大学动物医学院,湖北武汉,430070 2. 深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳,518001 3. 华中农业大学动物医学院,湖北武汉,430070 |
| 基金项目： | 国家863计划项目(2006AA10Z445);;中国博士后科学基金项目(20080430855) |
| 摘 要： | 根据已经扩增的鹿流行性出血病病毒(EHDV)VP7基因序列,设计一对扩增VP7基因特异性引物,用RT-PCR从血清I型EHDV(EHDV-1)总RNA中扩增VP7基因,并将其克隆到原核表达载体pBAD/Thio中,构建了重组原核表达质粒pBAD/Thio-EHDV VP7。将表达载体转入大肠埃希菌(E.coliLMG194),用L-阿拉伯糖诱导表达。SDS-PAGE和Western blot试验结果表明,EHDV VP7基因在LMG194中得到了表达,其表达产物可以与EHDV阳性血清特异性反应,说明该融和蛋白具有免疫学活性。 |
| 关 键 词： | 鹿流行性出血病病毒（EHDV） VP7基因 基因克隆 表达 |
Cloning and Prokaryotic Expression 0f VP7 Gene of Epizootic Hemorrhagic Disease Virus of Deer in E.coli |
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| YANG Jun-xing,HUA Qun-yi,CHEN Huan-chun,CHEN Bing,Lü Jiang-qiang,CAO Chen-fu,RUAN Zhou-xi,ZHANG Cai-hong.Cloning and Prokaryotic Expression 0f VP7 Gene of Epizootic Hemorrhagic Disease Virus of Deer in E.coli[J].Progress In Veterinary Medicine,2009,30(8):5-8. | |
| Authors: | YANG Jun-xing HUA Qun-yi CHEN Huan-chun CHEN Bing Lü Jiang-qiang CAO Chen-fu RUAN Zhou-xi ZHANG Cai-hong |
| Institution: | 1.Technic Centre of Animal and Plant Inspection and Quarantine;Shenzhen Entry-Exit Inspection and Quarantine Bureau;Shenzhen;Guangdong;518001;China;2.State Key Laboratory of Agricultural Microbiology;Huazhong Agricultural University;Wuhan;Hubei;430070;China |
| Abstract: | According to the published sequence in GenBank of VP7 gene of epizootic hemorrhagic disease of deer virus(EHDV),a pair of specific primers were designed and synthesized.The VP7 gene was amplified by RT-PCR from the total RNA extracted from EHDV.The PCR product was cloned into pBAD/Thio liner plasmid forming the recombinant expression vector pBAD/Thio-EHDV VP7.The pBAD-EHDV-VP7 was transformed into E.coli LMG194 and induced by L-arabinose to express recombinant protein.SDS-PAGE and Western blot indicated tha... |
| Keywords: | Epizootic hemorrhagic disease virus of deer VP7 protein gene cloning expression |
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