| 大孔吸附树脂分离富集铁筷子总皂苷的研究 |
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| 引用本文： | 张艳红,杨健,苗芳,尉耀元,周乐.大孔吸附树脂分离富集铁筷子总皂苷的研究[J].西北农业学报,2008,17(6):262-266. |
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| 作者姓名： | 张艳红 杨健 苗芳 尉耀元 周乐 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西杨凌,712100
2. 西北农林科技大学,理学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金
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| 摘 要： | 对7种大孔吸附树脂的铁筷子总皂苷吸附性能进行了比较筛选试验,并对选定的树脂进行了吸附与解吸优化条件研究。其中LSA-30型大孔吸附树脂具有较佳的吸附能力,其吸附分离铁筷子总皂苷的适宜条件为:上样浓度60 mg/mL,吸附流速2.0 BV/h,洗脱剂为体积分数30%~95%的乙醇,洗脱速度1.0 BV/h,NaCl]为0.5 mol/mL;95%乙醇洗脱液干燥后总固形物中铁筷子总皂苷含量达到60%以上。
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| 关 键 词： | 铁筷子 铁筷子总皂苷 大孔吸附树脂 |
| 收稿时间： | 2008/6/2 0:00:00 |
| 修稿时间： | 2008/6/10 0:00:00 |
Study on Separation and Purification of Total Saponins fromHelleborus thibetanus by Macroporous Adsorption Resin |
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| ZHANG Yan-hong,YANG Jian,MIAO Fang,WEI Yao-yuan and ZHOU Le.Study on Separation and Purification of Total Saponins fromHelleborus thibetanus by Macroporous Adsorption Resin[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(6):262-266. |
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| Authors: | ZHANG Yan-hong YANG Jian MIAO Fang WEI Yao-yuan and ZHOU Le |
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| Institution: | College of Life Sciences, Northwest A&F University, Yangling Shaanxi 712100, China;College of Science, Northwest A&F University, Yangling Shaanxi 712100, China;College of Life Sciences, Northwest A&F University, Yangling Shaanxi 712100, China;College of Science, Northwest A&F University, Yangling Shaanxi 712100, China;College of Science, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | Seven macroporous adsorption resins were compared on their adsorption properties for total saponins from Helleborus thibetanus,and the LSA-30 type resin possessed the better adsorption ability.The optimized conditions of adsorption and desorption were as following: the concentration of sample was 60 mg/mL;the current velocity of adsorption was 2.0BV/h;the eluting reagent was 30%~95% EtOH;eluting rate was 1.0 BV/h; was 0.5 mol/mL.The content of total saponins was up to 60% in the dried fraction of 95% ethanolic elution. |
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| Keywords: | LSA-30 |
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