|
|||||
|
|
| HPLC法测定帕那珠利的含量 | |
| 引用本文： | 王翔林.HPLC法测定帕那珠利的含量[J].中国兽药杂志,2012,46(8):35-38. |
| 作者姓名： | 王翔林 |
| 作者单位： | 1. 沈阳药科大学药学院,沈阳,110016 2. 辽宁省兽药饲料畜产品质量安全检测中心,沈阳,110016 3. 沈阳顺旺动物药业有限公司,沈阳,110300 |
| 摘 要： | 建立了帕那珠利原料药含量测定的HPLC法.以0.2％乙酸水溶液-乙腈(43∶57)为流动相,采用Diamonsil C18反相色谱柱(250 mm×4.6 mm,5μm),柱温35℃,流速1 mL/min,紫外检测波长255 nm.在此实验条件下,帕那珠利在10 ～ 100 μg/mL浓度范围内与峰面积线性关系良好(r=0.9999,n=5);精密度良好,RSD为0.25％(n=5);平均回收率为98.6％,RSD为1.2％.(n=9);重现性良好,RSD为0.69％(n=5);样品在24h内稳定,RSD为0.74％.本方法准确、简便、快速,可以用于帕那珠利的含量测定. |
| 关 键 词： | 帕那珠利 HPLC法 含量测定 |
| 收稿时间： | 3/7/2012 1:46:25 PM |
| 修稿时间： | 2012/6/18 0:00:00 |
Determination of ponazuril content by HPLC |
|
| WANG Xiang-lin , WANG Guan-nan , QIAN Chen , CHEN Chen , KANG Yu-zhu.Determination of ponazuril content by HPLC[J].Chinese Journal of Veterinary Drug,2012,46(8):35-38. | |
| Authors: | WANG Xiang-lin WANG Guan-nan QIAN Chen CHEN Chen KANG Yu-zhu |
| Institution: | School of Pharmacy,Shenyang Pharmaceutical University |
| Abstract: | The paper established an HPLC method for determination of ponazuril.The diamonsil C18 column was used;the mobile phase consisted of water(0.2% acetic acid)-acetonitrile(43∶ 57);the flow rate was 1 mL/min;the column temperature had been remained at 35 ℃;the detection wavelength was 255 nm.The linear range of ponazuril was 10~100 μg/mL(r=0.9999).The mean recovery was 98.6%(n=9).The precision was excellent(RSD=0.25%,n=5) as well as the reproducibility(RSD=0.69%,n=5).The sample was stable enough to be detected in 24 hours(RSD=0.74%).This method is accurate,easy and fast.It can be used to detect and quantify ponazuril. |
| Keywords: | ponazuril HPLC assay |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 | |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 | |