Application of 16S rDNA Sequence Analysis Technique in Microbial Detection |
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Authors: | Zhangyu LEI Xiao ZHANG |
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Institution: | (Hubei Ecology Vocational Colleague, Wuhan 430200, China) |
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Abstract: | With conserved regions and regions of high variations, 16s rDNA is an important molecular basis for the biological species identification and system evolu- tion. The modem molecular biology with 16s rDNA as the primer can accurately re- veal the diversity of microorganisms species and inheritance, thereby 16s rDNA se- quence analysis has become the main basis for classification and identification of microorganisms. Having overcome the limitations of traditional microculture methods, this method is easy to operate, quick and accurate to detect with high sensitivity, making it widely apply to species identification, community comparative analysis, phytecoenogenesis and the assessment of population diversity. It is a objective classification method with high credibility. |
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Keywords: | Microorganisms Classification and identification 16s rDNA 16s rRNA |
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