| 微波法测定分析鱼油脂成分(英文) |
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| 引用本文： | 彭德姣,刘祝祥,陈玲,朱杰英.微波法测定分析鱼油脂成分(英文)[J].农业科学与技术,2014(1):74-78. |
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| 作者姓名： | 彭德姣 刘祝祥 陈玲 朱杰英 |
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| 作者单位： | 吉首大学生物资源与环境科学学院,湖南吉首416000 |
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| 基金项目： | 湖南省科技厅项目（2010SK303）;吉首大学项目（JDSTLY201110）;国家科技型中小企业技术创新基金（12C26214304972） |
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| 摘 要： | 采用微波提取斑点叉尾鮰内脏鱼油,然后加酸使其甲酯化,以气相色谱/质谱法分析鱼油中的脂肪酸。结果表明斑点叉尾鮰内脏纯油脂中鱼油达到99%。从鱼油中共鉴定出23种成分,有饱和及不饱和脂肪酸,还有少量烷烃类物质。不饱和脂肪酸含量为72.84%,其中多不饱和脂肪酸为19.12%,以C18:2(16.54%)为主,单不饱和脂肪酸为53.72%,以C18:1(48.52%)为主。饱和脂肪酸含量为19.21%,主要有C16:0(14.46%)和C18:0(4.17%),多是低于C18以上的中长链脂肪酸。因此斑点叉尾鮰油脂可作功能性脂肪酸的重要膳食来源。该研究为探讨斑点叉尾鮰纯油脂成分构成,综合利用和开发斑点叉尾鮰鱼提供理论基础和质量控制方法
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| 关 键 词： | 斑点叉尾鮰鱼 鱼油 脂肪酸 GC-MS |
Determination and Analysis of Liposoluble Chemical Constituents from Ictalurus punctatus Oil |
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| Dejiao PENG,Zhuxiang LIU,Ling CHEN,Jieying ZHU.Determination and Analysis of Liposoluble Chemical Constituents from Ictalurus punctatus Oil[J].Agricultural Science ＆ Technology,2014(1):74-78. |
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| Authors: | Dejiao PENG Zhuxiang LIU Ling CHEN Jieying ZHU |
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| Institution: | (College of Biological Resources and Environmental Sciences, Jishou University 416000, China) |
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| Abstract: | The lipids was extracted from the internal organs of Ictalurus punctatus by microwave, and its liposoluble chemical constituents was identified and quantified by gas chromatography （GC）/electron impact （EI） mass spectrometry （MS）. Subsequent-ly, the acid catalysis method was used to convert fatty acids to methyl-esters. The total lipids in the pure fat of I. punctatus were found to be about 99%. Twenty-three fatty acids were identified. The major fatty acids found in the oils were normal mo-nounsaturated, polyunsaturated fatty acids and alkane paraffin. Unsaturated fatty acids comprised 72.84% of the total, and the major components of monounsaturated fatty acids were C18：1 （48.52%）, while the polyunsaturated fatty acids were mainly composed of C18：2 （16.54%）. Saturated fatty acids comprised approximately 19.21%of the total. The main components were C16：0 （14.46%） and C18：0 （4.17%）, most of which were medium-long-chain fatty acids. Therefore, catfish oil can be used as an important dietary source of functional fatty acids. The difference between the pro-portion of unsaturated and saturated fatty acids appears to be significant compared to fish of the Cyprinidae family, as catfish skin and muscle have higher protein content and lower fatty content. This study confirms that catfish has excellent nutri-tional quality. |
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| Keywords: | Ictalurus punctatus Fish oil Fatty acid GC-MS |
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