| 鸡源沙门氏菌多重耐药和Ⅰ型整合子的流行调查(英文) |
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| 引用本文： | 陆彦,赵红玉,张中文,候晓林,吴国娟.鸡源沙门氏菌多重耐药和Ⅰ型整合子的流行调查(英文)[J].农业科学与技术,2014(5):877-881. |
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| 作者姓名： | 陆彦 赵红玉 张中文 候晓林 吴国娟 |
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| 作者单位： | [1]北京农学院,北京102206 [2]北京市生命科学研究所,北京102206 |
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| 基金项目： | 国家自然科学基金（31201949,31172362）. |
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| 摘 要： | 目的]该研究旨在研究沙门氏菌多重耐药性与Ⅰ型整合子的流行情况。方法]该研究从山东省鸡场分离沙门氏菌,根据Kauffmann-White方法测定其血清型,采用微量稀释法测定了沙门氏菌对19种常用抗菌药物的MIC值,并且采用PCR技术测定了Ⅰ型整合子及其携带的耐药基因盒。结果]本次分离得到311株沙门氏菌,包括印地安纳沙门氏菌(133株)和肠炎沙门氏菌(178株)2种血清型;药物敏感性试验表明:分离菌株对磺胺嘧啶、磺胺甲恶唑、萘啶酸、氨苄西林、四环素、多西环素和甲氧苄啶普遍耐药,菌株多重耐药率为91.0%(283/311),99%的分离株对阿米卡星、多黏菌素敏感;PCR测定Ⅰ型整合子结果:Ⅰ型整合子的检出率为65.0%(202/311),其中多重耐药表型菌株Ⅰ型整合子阳性率为92.6%,202株整合子阳性菌株中有6株的携带基因盒dfr17-aadA5。结论]Ⅰ型整合子普遍存在于沙门氏菌中,并且与沙门氏菌的多重耐药性密切相关。
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| 关 键 词： | 沙门氏菌 多重耐药性 整合子 基因盒 |
Survey of Multidrug Resistance and Class I Integron Prevalence in Chicken-derived Salmonella |
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| Yan LU,Hongyu ZHAO,Zhongwen ZHANG,Xiaolin HOU,Guojuan WU.Survey of Multidrug Resistance and Class I Integron Prevalence in Chicken-derived Salmonella[J].Agricultural Science ＆ Technology,2014(5):877-881. |
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| Authors: | Yan LU Hongyu ZHAO Zhongwen ZHANG Xiaolin HOU Guojuan WU |
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| Institution: | 1. Beijing Agricultural College, Beijing 102206, China; 2. National Institute of Biological Sciences, Beijing 102206, China) |
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| Abstract: | Objective] This study aimed to investigate the multidrug resistance and prevalence of class I integrons in Salmonel a. Method] Salmonel a strains were isolated from chicken farms in Shandong Province. Kauffmann-White classification method was employed to analyze the serotypes of Salmonel a strains. Minimum in-hibition concentration （MIC） of Salmonel a strains against 19 common antimicrobial drugs was analyzed determined with microdilution method. The class I integrons and carried drug resistance gene cassettes were detected by PCR. Result] A total of 311 Salmonel a strains were isolated and classified into two serotypes, including 133 Salmonel a Indiana strains and 178 Salmonel a Enteritidis strains. Drug sensitivity test showed that the isolated Salmonel a strains were general y resistant to sulfadiazine, sulfamethoxazole, nalidixic acid, ampicil in, tetracycline, doxycycline and trimethoprim, with a multidrug resistance rate of 91.0% （283/311）; 99% strains were sensitive to amikacin and colistin. PCR assay indicated that the detection rate of class I integrons was 65.0% （202/311）; the positive rate of class I integrons in Salmonel a strains with multidrug resistance was 92.6%; among 202 positive strains, six strains carried gene cassette dfr17-aadA5. Conclusion] According to the above results, class I integrons exist general y in Salmonel a and are closely associated with the multidrug resistance of Salmonel a strains. |
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| Keywords: | Salmonella Multidrug resistance Class I integron gene cassette |
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