| Directional Breeding of An Oval-ecotype Male Sterile Line of Chinese Cabbage(Brassica campestris L. ssp. pekinensis) |
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| 作者姓名： | 谭翀 郭瑛琪 岳艳玲 |
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| 作者单位： | 云南农业大学园林园艺学院,云南昆 650201 |
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| 基金项目： | 基金项目国家自然科学基金（31101551）;云南省自然科学基金（2010CD057）：公益性行业（农业）科研专项（201003029） |
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| 摘 要： | 目的]探究大白菜定向转育卵圆生态型雄性不育系的方法。方法]以大白菜核不育＂复等位基因遗传假说＂为理论依据，以甲型两用系‘AB12’为不育供体品系，以卵圆生态型可育品系‘06048’为待转育品系，采用杂交、回交、兄妹交和测交的方法，按照定向转育方案将核不育基因定向转育。结果]各世代育性分离比率与理论相一致，成功育成了一种不育株率100%的具有‘06048’园艺性状的新核不育系，实现了核不育基因和园艺特性的同时转育。结论]该研究成果验证此定向转育方案是可行的，进一步为基因型为 msms的其他园艺性状的大白菜定向转育提供理论基础，也可以推广应用到整个芸薹属其它经济作物中，极大的拓宽了优良核不育基因的实际应用范围。
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| 关 键 词： | 大白菜 复等位基因核不育系 定向转育 |
Directional Breeding of An Oval-ecotype Male Sterile Line of Chinese Cabbage（ Brassica campestris L. ssp. pekinensis ） |
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| Chong TAN,Yingqi GUO,Yanling YUE.Directional Breeding of An Oval-ecotype Male Sterile Line of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)[J].Agricultural Science ＆ Technology,2014(3):333-337. |
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| Authors: | Chong TAN Yingqi GUO Yanling YUE |
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| Institution: | College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Objective] The study aimed to explore the method for directional breeding of a male-sterile line in oval-ecotype Chinese cabbage. Method] Based on ＂Multiple Allele Hypothesis of Genic Male Sterile Chinese Cabbage＂, an inbred line ＇06048＇ of oval ecotype was used as the receptor, and male fertile plant of ＇AB12＇ was used as the donor line. Crossing, backcross, selfing, testcross and sibling were ap- plied to transfer the multiple alleles under the directional genetic model. Result] Segregation ratio of every generation was consistent with theoretical value. A new male sterile line with 100% male sterility and ＇06048＇ horticultural traits was ob- tained successfully, which accomplished the transfer of male sterile multiple allele and horticultural characters of receptor line at the same time. Conclusion] The re- search verifies that the model of directional transfer is feasible, provides a theoreti- cal basis for the directional transfer of Chinese cabbage with other horticultural traits whose genotype is msms. The model can also be applied to other Brassica crops, to generate genetic male sterile lines with specific botanical traits and high-quality economic traits. |
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| Keywords: | Chinese cabbage Multiple-allele genic male sterility Directional transfer |
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