| 牛眼球石蜡切片制作方法的探索(英文) |
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| 引用本文： | 邓立新,郭延俭,王绿林,王朝阳,董海聚,李杨,贺丛.牛眼球石蜡切片制作方法的探索(英文)[J].农业科学与技术,2014(1):71-73. |
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| 作者姓名： | 邓立新 郭延俭 王绿林 王朝阳 董海聚 李杨 贺丛 |
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| 作者单位： | [1]河南农业大学牧医工程学院,河南郑州450002 [2]山东农业大学动物科技学院,山东泰安271000 |
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| 基金项目： | 现代农业（肉牛牦牛）产业技术体系专项资金资助（CARS-38） |
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| 摘 要： | 目的]探索一种牛眼球的石蜡切片制作方法。方法]试验对照组采用常规石蜡切片制作,试验组采用"角膜开窗再固定法"制作石蜡切片。结果]试验组标本固定后肉眼可见视网膜与脉络膜紧密相连,仅有小部分视网膜脱离的现象;切片显示小梁网连续性未被破坏、眼球壁全层组织连续性未破坏、各层未出现分离现象;视网膜无脱离、各层组织细胞无收缩现象。说明试验组大大改进了眼球固定、脱水、透明技术。结论]为眼球研究和相应病理研究提供基础。
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| 关 键 词： | 牛 眼球 石蜡切片 角膜开窗再固定法 |
Exploration of Methods for Preparation of Cattle Eyeball Paraffin Sections |
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| Lixin DENG,Yanjian GUO,Lvlin WANG,Chaoyang WANG,Haiju DONG,Yang LI,Cong HE.Exploration of Methods for Preparation of Cattle Eyeball Paraffin Sections[J].Agricultural Science ＆ Technology,2014(1):71-73. |
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| Authors: | Lixin DENG Yanjian GUO Lvlin WANG Chaoyang WANG Haiju DONG Yang LI Cong HE |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; 2. College of Animal Science and Veterinary Medicine, Shangdong Agricultural University, Tai＇an 271000, China) |
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| Abstract: | Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed＆quot;opening a window on cornea and refixation＆quot; was used to prepare paraffin sections for cattle eyebal s in the treatment group. Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s. |
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| Keywords: | Cattle Eyeball Paraffin sections ＂Opening a window on cornea andrefixation＂ method |
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