| 长柄石杉中石杉碱甲的含量测定 |
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| 引用本文： | 张敬杰,李齐激,潘炉台.长柄石杉中石杉碱甲的含量测定[J].安徽农业科学,2012,40(2):726+757. |
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| 作者姓名： | 张敬杰 李齐激 潘炉台 |
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| 作者单位： | 贵阳中医学院,贵州贵阳,550002;贵阳中医学院,贵州贵阳,550002;贵阳中医学院,贵州贵阳,550002 |
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| 基金项目： | 贵州省科技计划项目:黔科合GY字[2008]3038 |
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| 摘 要： | 目的]建立HPLC法测定长柄石杉中石杉碱甲的含量。方法]色谱条件:色谱柱为ZORBAX Extend-C18(150 mm×4.6 mm,4.5μm),流动相为乙腈-浓度0.02 mol/L磷酸二氢钾(8∶92,V/V),检测波长为310 nm,柱温为25℃,流速为1 ml/min,进样量为10μl。结果]石杉碱甲在0.046～0.460μg/ml浓度范围内与峰面积呈良好线性关系(R=0.999 9),平均回收率为97.45%,RSD为2.70%,平均含量为0.025%。结论]该方法简单、易行,可用于长柄石杉中石杉碱甲的含量测定。
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| 关 键 词： | 长柄石杉(Huperzia serrata(Thunb.)Trev.forma longipetiolata(Spring) Ching) 石杉碱甲 含量测定 |
Determination on Huperzine A Content in Huperzia serrata(Thunb.) Trev. forma longipetiolata(Spring) Ching |
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| Institution: | ZHANG Jing-jie et al(Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002) |
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| Abstract: | Objective] To develop a HPLC method for determining huperzine A content in uperzia serrata(Thunb.) Trev.forma longipetiolata.Methods] The chromatographic conditions: chromatographic column: ZORBAX Extend-C18(150 mm×4.6 mm,4.5 μm),mobile phase of 0.02 mol/L KH2PO4-CH3CN(92∶8,V/V),detection wavelength: 310 nm,column temperature: 25 ℃,flow velocity of 1 ml/min,sample size of 10 μl.Results] The calibration curves of huperzine A showed good linearity in the range of 0.046-0.460 μg/ml with a correlation coefficient of 0.999 9,the average recovery was 97.45%,with RSD of 2.70%,and the average content was 0.025%.Conclusion] The method is simple and easy,can be used for the determination of huperzine A content in Huperzia serrata(Thunb.) Trev.forma longipetiolata. |
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| Keywords: | Huperzia serrata(Thunb ) Trev forma longipetiolata Huperzine A Content determination |
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