| 钙调素mRNA在梨子房和幼果中的表达 |
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| 引用本文： | 岳海林,邓秀新,彭抒昂.钙调素mRNA在梨子房和幼果中的表达[J].中国农业科学,2008,41(1):176-181. |
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| 作者姓名： | 岳海林 邓秀新 彭抒昂 |
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| 作者单位： | 1. 华中农业大学作物遗传改良国家重点实验室,武汉,430070;华中农业大学园艺植物生物学教育部重点实验室,武汉,430070
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金
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教育部创新团队发展基金 |
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| 摘 要： | 【目的】通过研究钙调素mRNA在梨（Pyrus pyrifolia NaKai）子房和幼果中的表达特性，探讨其在盛花后子房和幼果发育中的生理作用。【方法】以10年生黄花梨盛花前后子房和幼果为试材。Northern杂交检测钙调素基因表达水平。mRNA原位杂交进一步检测钙调素mRNA在梨子房和幼果发育过程中组织特异性表达。【结果】Northern杂交结果表明，钙调素基因在梨子房和幼果中的表达水平，花前逐渐上升，至盛花时最高，花后又下降。mRNA原位杂交结果表明，钙调素mRNA在梨果中的表达多集中于果皮、果肉、胚珠和果肉微管组织，而在果心的表达较少，且表达水平为果皮>果肉>果心，珠心>胚囊>珠被。在细胞水平上，钙调素mRNA的表达主要集中于细胞的胞间隙和胞间层及细胞核；钙调素mRNA的表达水平在各组织不同发育阶段也表现为盛花时最高，花前稍高于花后。【结论】钙调素mRNA在梨子房和幼果中大量表达，以盛花期最高，表达的部位主要集中在果皮、果肉、胚珠、维管束、细胞间隙及胞间层。钙调素mRNA在盛花期子房/幼果中的大量表达，可能与果实钙的增加有一定关系。
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| 关 键 词： | 钙调素mRNA 原位杂交 子房/幼果 梨 |
| 收稿时间： | 2007-01-31 |
| 修稿时间： | 2007-04-02 |
Expression of Calmodulin mRNAs in Ovaries and Fruitlets of Pear |
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| YUE Hai-lin,DENG Xiu-xin,PENG Shu-ang.Expression of Calmodulin mRNAs in Ovaries and Fruitlets of Pear[J].Scientia Agricultura Sinica,2008,41(1):176-181. |
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| Authors: | YUE Hai-lin DENG Xiu-xin PENG Shu-ang |
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| Abstract: | Abstract: 【Objective】To investigate the temporal and spatial expression pattern of calmodulin mRNA in pear ovaries and fruitlets, this study aimed to elucidate the mechanism of how fertilization promotes calcium uptake, increase calmodulin content and interaction between them.【Method】The ovaries and fruitlets before and after flowering of ten-year-old ‘Huanghua’ pear(Pyrus pyrifolia NaKai) were investigated, via northern hybridization and mRNA in situ hybridization for the expression level and localization of calmodulin gene .【Result】Results demonstrated that the expression level of calmodulin gene increased gradually before flowering, reached the peak at full blossom, and then decreased after flowering. mRNA in situ hybridization showed that calmodulin mRNA located on the epidermis, sarcocarp, ovule and flesh vascular bundles，while there was no expression at core and endocarp. The expression levels of calmodulin mRNA were as follows from high to low :epidermis , sarcocarp and endocarp; nucellus, embryonic sac and integument. At cellular level, calmodulin mRNA located at nucleolus, interstitial space and intercellular layer. With fruit developing, the expression level in different tissues showed that the highest level was at full blossom, and the expression level was slightly higher before blossom than that after blossom.【Conclusion】As showed that calmodulin mRNA expressed highly in flesh and ovule at blossom, and promoted calcium uptake. |
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| Keywords: | Calmodulin mRNA in situ hybridization ovary/fruitlet |
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