| 刃天青显色法检测铜绿假单胞菌对几种抗菌药物的敏感性 |
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| 引用本文： | 李宙阳,周英.刃天青显色法检测铜绿假单胞菌对几种抗菌药物的敏感性[J].贵州大学学报(农业与生物科学版),2010,29(3):237-241. |
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| 作者姓名： | 李宙阳 周英 |
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| 作者单位： | 贵州大学,生命科学学院,贵州,贵阳,550025 |
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| 基金项目： | 贵州省中药现代化专项 |
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| 摘 要： | 采用刃天青显色法检测3种抗菌药物(头孢哌酮钠、链霉素、青霉素)对铜绿假单胞菌的MIC和最佳作用时间,并比较与微量稀释法的相关性,探讨应用该法的可行性。结果表明:刃天青的试验浓度在75~150μg/mL为宜,细菌接种量1×105cfu/孔,肉眼即可判定MIC值结果,判定时间为8 h。3种抗菌药物对铜绿假单胞菌的MIC和最佳作用时间分别为:5μg/mL,4~6 h;20μg/mL,6~8 h;不敏感。刃天青显色法与稀释法MIC值检测结果一致。本试验结果说明,刃天青显色法具有简便、快速、灵敏度高的特点,能动态地反映细胞随时间变化的生长情况,能检测药物的最佳作用时间。
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| 关 键 词： | 刃天青 铜绿假单胞菌 MIC 最佳作用时间 |
A Resazurin-based Assay for Evaluation of Anti-pseudomonas Aeruginosa Drugs Sensitivity |
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| LI Zhou-yang,ZHOU Ying.A Resazurin-based Assay for Evaluation of Anti-pseudomonas Aeruginosa Drugs Sensitivity[J].Journal of Mountain Agriculture & Biology,2010,29(3):237-241. |
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| Authors: | LI Zhou-yang ZHOU Ying |
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| Institution: | ( College of Life Sciences, Guizhou University, Guiyang Guizhou 550025, China) |
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| Abstract: | A resazurin-based assay was employed to evaluate MIC and the optimal function time of anti- P. vaeruginosa drugs in the current work. The results demonstrated that under the optimal condition of 75 - 150 ug/mL of resazurin, 1 × 105 cfu/well of bacteria inoculation density, and 8 hours of incubation, the MIC could be judged by naked eye. The MIC for cefoperazone sodium, streptomycin, or penicillin were 5 ug/ml, 20 ug/ml or un-sensitive, respectively. The optimal function times for cefoperazone sodium and streptomycin were 4 -6 h, 6 -8 h, respectively. The results from the resazurin -based assay were consistent with that obtained using conventional dilution assay. Therefore, resazurin assay may dramatically detect the cell growth and evaluate MIC and the optimal function time of anti-bacteria drugs. This resazurin -assay has the considerable advantages to the other assays in both simplicity and convenience. |
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| Keywords: | MIC |
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