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| 新城疫病毒F和HN基因真核表达载体的构建 | |
| 引用本文： | 闻晓波,宋佰芬,姜海芳,崔玉东.新城疫病毒F和HN基因真核表达载体的构建[J].黑龙江八一农垦大学学报,2007,19(4):54-57. |
| 作者姓名： | 闻晓波 宋佰芬 姜海芳 崔玉东 |
| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,大庆,163319 2. 黑龙江八一农垦大学生命科技学院 3. 黑龙江八一农垦大学动物科技学院,大庆,163319;黑龙江八一农垦大学生命科技学院 |
| 摘 要： | 根据GenBank中新城疫病毒(NDV)F、HN基因序列和真核细胞表达载体pEGPF-N1的克隆位点,分别设计一对引物,通过PCR方法获得了F、HN基因,经回收纯化,将产物连接到克隆载体pMD18-T上。酶切鉴定和序列分析后,再将目的基因亚克隆到真核细胞表达载体pEGPF-N1上,经鉴定,成功构建含有NDVF和HN基因的真核细胞表达载体pEGPF-N1-F和pEGPF-N1-HN,为下一步研究NDVF和HN蛋白在细胞凋亡中的生物学功能奠定基础。 |
| 关 键 词： | 新城疫病毒 F基因 HN基因 真核细胞表达载体 |
| 文章编号： | 1002-2090（2007）04-0054-04 |
| 收稿时间： | 2007-07-21 |
| 修稿时间： | 2007年7月21日 |
Construction of eukaryotic expression vector harbouring F and HN gene of Newcastle disease virus |
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| WEN Xiao-bo,SONG Bai-fen,JIANG Hai-fang,Cui Yu-dong.Construction of eukaryotic expression vector harbouring F and HN gene of Newcastle disease virus[J].Journal of Heilongjiang August First Land Reclamation University,2007,19(4):54-57. | |
| Authors: | WEN Xiao-bo SONG Bai-fen JIANG Hai-fang Cui Yu-dong |
| Abstract: | According to the encoding sequence of the NDV F and HN protein and the multiple Cloning sites characteristic of the eukaryotic expressional vector pEGPF-N1 in GenBank, appropriate primers specific to NDV F and HN gene were designed. The target DNA fragments were obtained by PCR amplification and then were cloned into the pMD18-T vector respectively. After identification by restriction digestion and sequencing analysis, the specific DNA fragments were cloned into the eukaryotic expression vector pEGPF-N1, respectively. The positive recombinant plasmids were named pEGPF-N1-F and pEGPF-N1-HN after identification by restriction enzyme digestion, PCR amplification. Results showed that recombinant expression plasmids pEGPF-N1-F and pEGPF-N1-HN were successfully constructed and could be the foundation of analyzing biological role of F and HN protein of NDV in cell apoptosis. |
| Keywords: | Newcastle disease F gene HN gene eukaryotic cell expression vector |
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