| 结晶纤维素降解细菌的筛选、分离与鉴定 |
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| 引用本文： | 陈春岚,庞浩,唐纪良,冯家勋.结晶纤维素降解细菌的筛选、分离与鉴定[J].广西农业生物科学,2008,27(4):360-364. |
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| 作者姓名： | 陈春岚 庞浩 唐纪良 冯家勋 |
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| 作者单位： | 1. 广西大学,生命科学与技术学院,广西,南宁,530005;广西亚热带生物资源保护利用重点实验室,广西,南宁,530005;贺州学院,广西,贺州,542800
2. 广西大学,生命科学与技术学院,广西,南宁,530005;广西亚热带生物资源保护利用重点实验室,广西,南宁,530005 |
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| 基金项目： | 国家自然科学基金(30560003); 教育部新世纪优秀人才支持计划(NCET-05-0752); 广西科技攻关计划(桂科攻0630003-7) |
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| 摘 要： | 采用以结晶纤维素（Avicel）为唯一碳源的平板活性筛选法，从60份森林腐殖土、腐术样品中分离得到2株在pH5．0、37℃条件下高效降解结晶纤维素的细菌菌株GXN152和GXN153。以菌株的总DNA基因为模板，用细菌的16S rRNA基因的通用引物扩增得到菌株GXN152和GXN153的16S rRNA基因序列。测序分析表明菌株GXN152的16S rRNA基因序列和洋葱假单胞菌（Burkholderia cepacia）菌株CNR22的16S rRNA基因序列的同源性最高，具有98％的同源性，生理生化特性检测表明菌株GXN152具有洋葱假单胞菌的鉴别特征，将结晶纤维素降解菌GXN152鉴定为洋葱假单胞菌。菌株GXN153的16S rRNA基因序列和类芽孢杆菌（Paenibacillus favisporus）菌株GMP01的16S rRNA基因序列的同源性最高，具有99％的同源性，生理生化特性检测表明菌株GXN153具有类芽孢杆菌的鉴别特征，将纤维素降解菌GXN153鉴定为类芽孢杆菌。
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| 关 键 词： | 结晶纤维素 降解 细菌 筛选 鉴定 |
Screening,isolation and identification of bacterial strains of degrading crystalline cellulose |
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| CHEN Chun-lan,PANG Hao,TANG Ji-liang,FENG Jia-xun.Screening,isolation and identification of bacterial strains of degrading crystalline cellulose[J].Journal of Guangxi Agricultural and Biological Science,2008,27(4):360-364. |
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| Authors: | CHEN Chun-lan PANG Hao TANG Ji-liang FENG Jia-xun |
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| Institution: | CHEN Chun-lan , PANG Hao, TANG Ji-liang , FENG Jia-xun (1. College of Life Science and Technology, Guangxi University, Nanning 530005, China; 2. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530005, China; 3 .Hezhou University, Hezhou 42800, China) |
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| Abstract: | A total of 60 samples was collected from forest soils and dead woods and bacterial strains that can utilize crystalline cellulose Avicel as sole carbon at pH 5.0. 37℃ was isolated from these samples and screened on plates. Two bacterial strains, GXN152 and GXN153, were found to produce highest cellulases activity on plates. The 16S rRNA gene sequences were amplified from total DNA of strains GXN152 and GXN153 and were sequenced and analyzed. Results showed that the amplified 16S rRNA gene sequence of GXN152 shared highest homology （98% identity） with that of Burkholderia cepacia strain CNR22 and the 16S rRNA gene sequence of GXN153 shared highest homology （99 % identity） with that of Paenibacillus favisporus strain GMP01. The physiological and biochemical characteristics of GXN152 and GXN153 were characterized. It turned out that strain GXN152 possessed the essential characteristics of Burkholderia cepacia and strain GXN153 showed the typical properties of Paenibacillus favisporus. Thus, strain GXN152 was identified as Burkholderia cepacia and strain GXN153 was identified as Paenibacillus favisporus. |
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| Keywords: | Avicel degradation bacteria isolation identification |
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