| 根癌农杆菌介导BDN1 基因转化马铃薯的研究 |
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| 引用本文： | 潘映雪,张丽莉,石瑛,卢翠华,林忠平.根癌农杆菌介导BDN1 基因转化马铃薯的研究[J].中国蔬菜,2013,1(6):37-43. |
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| 作者姓名： | 潘映雪 张丽莉 石瑛 卢翠华 林忠平 |
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| 作者单位： | （1 东北农业大学农学院，黑龙江哈尔滨 150030；2 北京大学生命科学学院，北京 100871） |
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| 基金项目： | 黑龙江省科技计划项目(GZ11B103);国家高技术研究发展“863”计划项目(2009AA10Z103) |
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| 摘 要： | 以马铃薯品种东农303 微型薯为试验材料，通过根癌农杆菌介导法，将来自耐旱植物厚 叶旋
蒴苣苔的脱水蛋白基因BDN1 导入马铃 薯，并对其遗传转化的影响因素进行了研究。获得了抗性再生植株
35 株 ；PCR 检测27 株呈阳性；Southern-blot 检测4 株呈阳性，表明BDN1 基因已整合到马铃薯基因组中；
半定量RT-PCR 检测结果表明BDN1 基因在马铃薯抗性植株中表 达。通过对相对含水量、脯氨酸、丙二醛
含量和SOD 活性测定，证明4 个转基因株系对干旱胁迫的抗性显著高于对照植株。
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| 关 键 词： | 马铃薯 农杆菌介导 BDN1 基因 抗旱 遗传转化 |
| 收稿时间： | 2012-11-28; |
Studies on Potato Transformation of BDN1 Gene Mediated by Agrobacterium tumefaciens |
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| PAN Ying-xue,ZHANG Li-li,SHI Ying,LU Cui-hua,LIN Zhong-ping.Studies on Potato Transformation of BDN1 Gene Mediated by Agrobacterium tumefaciens[J].China Vegetables,2013,1(6):37-43. |
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| Authors: | PAN Ying-xue ZHANG Li-li SHI Ying LU Cui-hua LIN Zhong-ping |
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| Institution: | 1.College of Agronomy， Northeast Agricultural University， Harbin 150030， Heilongjiang, China； 2College of Life;
Science， Peking University， Beijing 100871， China） |
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| Abstract: | The BDN1 gene in coding area from drought-tolerant Boea crassifolia Hemsl was
transferred into potato（Solanum tuberosum L.） cultivars‘ Dongnong 303’ through agro-bacteriummediated
transformation. The factors influencing its genetic transformation were studied. Thirty-five
transgenic plants with kanamycin resistance were obtained. Specific primers were used to amplify the full
length of the BDN1 gene from total genomic DNA of the transgenic plants. Among them, 27 plants were
positive by PCR test and 4 plants were positive by Southern-blot test. The result of semi-quantitative
RT-PCR test showed that BDN1 gene was inserted into the genome of the transgenic plants and can be
transcribed normally. The physiological index showed the transgenic potato plants were significantly
tolerant to drought stress. |
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| Keywords: | Agrobacterium-mediated BDN1 gene BDN1 gene Drought-tolerant transformation Genetic
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