| 甘肃地区番茄细菌性斑点病菌的鉴定与检测 |
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| 引用本文： | 文朝慧,王军平,何苏琴.甘肃地区番茄细菌性斑点病菌的鉴定与检测[J].中国蔬菜,2013,1(6):68-73. |
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| 作者姓名： | 文朝慧 王军平 何苏琴 |
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| 作者单位： | （;1.甘肃出入境检验检疫局，甘肃兰州 730020；;2. 甘肃省农业科学院植物保护研究所，甘肃兰州;
730070） |
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| 基金项目： | 甘肃出入境检验检疫局科技计划项目(2011GK001) |
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| 摘 要： | 从番茄病果上分离纯化的细菌菌株，经致病性测定、形态特征、培养特性观察及16S rDNA
序列测定，鉴定为番茄细菌性斑点病菌Pseudomonas syringae pv. tomato（Okabe）Young，Dye et Wilkie。
利用特异性引物对已鉴定菌株及番茄发病组织进行PCR 扩增，均扩增出530 bp 左右的特异性片段，印证
了可利用特异性引物MM5F/MM5R 对分离菌株或番茄发病组织进行PCR 扩增以检测番茄细菌性斑点病菌。
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| 关 键 词： | 番茄细菌性斑点病菌 分离 检测 |
| 收稿时间： | 2012-11-20; |
Identification and Detection of Pseudomonas syringae pv.tomato in Gansu |
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| WEN Zhao-hui,WANG Jun-ping,HE Su-qin.Identification and Detection of Pseudomonas syringae pv.tomato in Gansu[J].China Vegetables,2013,1(6):68-73. |
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| Authors: | WEN Zhao-hui WANG Jun-ping HE Su-qin |
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| Institution: | （;1.Gansu Entry-exit Inspection and Quarantine Bureau， Lanzhou 730020， Gansu， China； ;2.Institute of Plant Protection，;
Gansu Academy of Agricultural Sciences， Lanzhou 730070， Gansu， China） |
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| Abstract: | Bacteria strains isolated from diseased tomato fruits were identified as Pseudomonas
syringae pv. tomato based on pathogenicity，morphological characterizations and 16S ribosomal DNA
sequence analysis（ 99% nucleotide identity）．About 530 bp specific fragment of hrpZPst were amplified
from these strains and naturally-infected tomato fruits by specific primers MM5F/MM5R．The results
confirm that this PCR protocol is suitable for specific detecti on of P. syringae pv. tomato in field material. |
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| Keywords: | Isolation Detection |
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