| 辽宁省甜瓜新病害——果腐病病原菌生物学特性研究 |
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| 引用本文： | 侯悦,刘志恒,曹友文,赵廷昌.辽宁省甜瓜新病害——果腐病病原菌生物学特性研究[J].中国蔬菜,2013,1(6):74-80. |
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| 作者姓名： | 侯悦 刘志恒 曹友文 赵廷昌 |
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| 作者单位： | （;1.沈阳农业大学植物保护学院，辽宁沈阳 110161；;2. 中国农业科学院植物保护研究所，北京 100193） |
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| 基金项目： | 国家西瓜甜瓜产业技术体系项目(CARS) |
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| 摘 要： | 针对辽宁省甜瓜新病害--茄丝核菌（Rhizoctonia solani）引起的果腐病，对其病原菌进行
了生物学测定。结果表明：病原菌菌丝生长以甜瓜煎汁培养基为最适；菌丝在5～40 ℃范围内均能生长，
最适温度为28 ℃ ；最适pH 值为7；光照对菌丝生长影响较小；适宜菌丝生长的碳、氮源分别为可溶性
淀粉和蛋白胨。菌核形成的适宜条件为25 ℃，pH 值6～8，理查培养基，最适碳源为山梨醇，最适氮源
为KNO3。菌核在10 种供试营养物质中均能萌发；在15～40 ℃范围内菌核均可萌发，28 ℃为最适温度，
黑暗条件有利于菌核萌发；以麦芽糖为碳源的PA 培养基和以蛋白胨为氮源的查氏培养基有利于菌核萌发。
菌丝致死温度为50 ℃、10 min。菌核致死温度为53 ℃、10 min。
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| 关 键 词： | 甜瓜 腐病 茄丝核菌 生物学特性 |
| 收稿时间： | 2012-10-29; |
Studies on Biological Characteristics of Fruit Rot Pathogen—A New Muskmelon Disease in Liaoning Province |
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| HOU Yue,LIU Zhi-heng,CAO You-wen,ZHAO Ting-chang.Studies on Biological Characteristics of Fruit Rot Pathogen—A New Muskmelon Disease in Liaoning Province[J].China Vegetables,2013,1(6):74-80. |
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| Authors: | HOU Yue LIU Zhi-heng CAO You-wen ZHAO Ting-chang |
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| Institution: | （;1.College of Plant Protection， Shenyang Agricultual University， Shenyang 110161， Liaoning， China； ;2.Institute of;
Plant Protection， Chinese Academy of Agricultural Sciences， Beijing 100193， China） |
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| Abstract: | The pathogen of muskmelon fruit rot was isolated from the samples of muskmelon from
Liaoning Province．The casual agent was identified as AG-4 HGI of Rhizoctonia solani by the studies
on morphology，pathogenicity，anastomosis groups and rDNA-ITS．The pathogenic biological characteristics
of muskmelon fruit rot were studied in this paper．The result showed that the best medium for
mycelial growth was Muskmelon juice culture medium．The temperature ranged for mycelial growth was
5-40 °C，28 °C was the optimum；the best pH was 7．The effect of light on mycelial growth was not
evident. Mycelial growth was in favor of soluble starch and Peptone．The ideal sclerotia formation was at
25 °C，pH 6-8，Richard culture medium．The suitable carbon source for the formation of sclerotia was
Sorbitol．The beneficial nitrogen source for the formation of sclerotia was KNO3．The sclerotia could
germinate in 10 kinds of nutrition．The proper temperature was 15-40 ℃，and 28 ℃ was the optimum.
Dark condition was conductive to sclerotium germination．The best carbon source and nitrogen source
for sclerotium germination are Malt Sugar and Peptone．Mycelial lethal temperature was 50 ℃，10 min．
The sclerotia lethal temperature was 53℃，10 min. |
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| Keywords: | Fruit rot Rhizoctonia solani Rhizoctonia solani Biological characteristics |
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