| 大蒜种质超低温保存及脱毒技术研究进展 |
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| 引用本文： | 刘晓雪,程智慧.大蒜种质超低温保存及脱毒技术研究进展[J].中国蔬菜,2013,1(2):12-19. |
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| 作者姓名： | 刘晓雪 程智慧 |
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| 作者单位： | 西北农林科技大学园艺学院，陕西杨凌 712100 |
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| 基金项目： | 国家公益性行业(农业)科研专项(200903018-7);国家自然科学基金(31171949) |
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| 摘 要： | 大蒜在每年的营养繁殖过程中，容易感染病毒并积累在鳞茎中随世代传递，引发病毒病，导
致大蒜种性退化，鳞茎商品品质降低。与传统脱毒方法相比，超低温法具有植株脱毒率高、操作简便、再
生植株遗传稳定性好、增殖率高且能与超低温保存种质结合利用等明显优势。本文主要综述了大蒜种质超
低温保存及其脱毒原理、研究现状与存在问题，并展望了超低温在大蒜种质保存方面的研究方向及超低温
处理应用于大蒜种质脱毒的潜力。
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| 关 键 词： | 大蒜 超低温保存 脱毒 问题与展望 综述 |
| 收稿时间： | 2012-09-03; |
Research Progress on Cryopreservation of Garlic Germplasm and Its Virus Eradication Technique |
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| LIU Xiao-xue,CHENG Zhi-hui.Research Progress on Cryopreservation of Garlic Germplasm and Its Virus Eradication Technique[J].China Vegetables,2013,1(2):12-19. |
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| Authors: | LIU Xiao-xue CHENG Zhi-hui |
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| Institution: | College of Horticulture， Northwest A & F University， Yangling 712 100， Shaanxi， China |
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| Abstract: | Garlic（Allium sativum L.） is susceptible to virus during its annual vegetative
propagation and the pathogens accumulated in bulbs are transmitted to the next generations，resulting
in garlic virus disease．Virus infection will lead to garlic degeneration，and reduce the quality of
bulbs．Compared with the traditional detoxification metho ds，ultra-low temperature method has
many advantages，such as higher virus-free rate and easy operation，better genetic stability，higher
proliferation rate．Besides，it can be combined with germplasm cryopreservation．This article
mainly summarizes the principles of cryopreservation and detoxification of garlic germplasm，present
status in research，and existing problems．The article also prospects the research direction of using
cryopreservation to preserve garlic germplasm and the potential of applying cryotherapy for detoxification
of garlic germplasm. |
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| Keywords: | Cryopreservation Detoxification Problems and prospects Review |
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