| Effects of gibberellin and its inhibitors on growth and gene expression in Poa pratensis北大核心CSCD |
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| 引用本文： | 赵利清,郝志刚,崔笑岩,彭向永.Effects of gibberellin and its inhibitors on growth and gene expression in Poa pratensis北大核心CSCD[J].草业学报,2022,31(3):85-91. |
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| 作者姓名： | 赵利清 郝志刚 崔笑岩 彭向永 |
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| 作者单位： | 1.国家开放大学，北京 100039;2.银河生态科技有限公司，北京 102488;3.曲阜师范大学生命科学学院，山东 曲阜 273165 |
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| 基金项目： | 曲阜师范大学校企合作课题 |
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| 摘 要： | 研究了不同浓度的赤霉素及其抑制剂烯效唑和多效唑对草地早熟禾生长及赤霉素相关基因表达的影响，为外源生长调节剂在草坪养护管理中的高效利用提供理论依据。以1/2 Hogland营养液培养的草地早熟禾为材料，随机分成7组，分别施用不同浓度的赤霉素、烯效唑、多效唑，测定根及叶片的生长量、叶绿体分布、叶绿素含量以及赤霉素相关基因的表达量。结果显示：与对照比较，外源烯效唑和多效唑均显著抑制草地早熟禾的伸长生长以及干/鲜重，但提高了根冠比、叶绿素a、叶绿素b及类胡萝卜素含量（P<0.05）；增加了叶肉细胞层数，使叶绿体充满在叶片上下表皮之间的叶肉细胞中；赤霉素合成代谢途径关键酶基因PpKAO、PpKS1、PpGA3ox、PpGA2ox表达量显著下调，赤霉素信号转导受体蛋白基因PpGID1显著上调（P<0.05）。外源赤霉素对草地早熟禾的伸长生长具有促进作用，但降低了根、叶的干/鲜重，提高根冠比，显著提高了赤霉素分解基因PpGA2ox表达量（P<0.05）。总之，烯效唑、多效唑对草地早熟禾生长发育及基因表达的调控作用与赤霉素相反，但无明显浓度效应；在草坪修剪后立即喷施1000倍的烯效唑或多效唑，对减少用工成本，增加草坪观赏性、提高园林绿化中的草坪草管护水平具有重要意义。
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| 关 键 词： | 草地早熟禾 生长调节剂 赤霉素 生长 基因表达 |
| 收稿时间： | 2020-12-09 |
| 修稿时间： | 2021-03-04 |
Effects of gibberellin and its inhibitors on growth and gene expression in Poa pratensis |
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| ZHAO Li-qing,HAO Zhi-gang,CUI Xiao-yan,PENG Xiang-yong.Effects of gibberellin and its inhibitors on growth and gene expression in Poa pratensis[J].Acta Prataculturae Sinica,2022,31(3):85-91. |
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| Authors: | ZHAO Li-qing HAO Zhi-gang CUI Xiao-yan PENG Xiang-yong |
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| Institution: | 1.The Open University of China，Beijing 100039，China;2.Yinhe Ecology Technology Co. ，Ltd，Beijing 102488，China;3.College of Life Sciences Qufu Normal University，Qufu 273165，China |
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| Abstract: | The effects of different concentrations of gibberellin （GA3） and gibberellin inhibitors on the growth and development， and the expression of gibberellin-related genes of Poa pratensis were studied. P. pratensis plants cultivated with 1/2 Hoagland nutrient solution were randomly divided into 7 groups. Three plant growth regulators： GA3， uniconazole or paclobutrazol were applied to determine the effects on plant growth， chloroplast distribution， chlorophyll content， and gibberellin-related gene expression measured. Compared with the control， exogenous uniconazole and paclobutrazol significantly inhibited leaf elongation and fresh and dry weight accumulation of P. pratensis， but increased root∶shoot ratio， chlorophyll a， chlorophyll b and carotenoid contents （P<0.05）. The number of mesophyll cell layers was also increased and Chloroplasts were evenly distributed in mesophyll cells between the upper and lower epidermis. The expression of key enzyme genes PpKAO， PpKS1， PpGA3ox and PpGA2ox in the gibberellin biosynthesis and metabolism pathways were significantly down-regulated， and the protein receptor gene PpGID1 in the gibberellin signal transduction pathway was significantly up-regulated （P<0.05）. Exogenous gibberellin promoted the elongation of P. pratensis roots and leaves， and increased the fresh and dry weights of roots and leaves， decreased the root∶shoot ratio and significantly upregulated the PpGA2ox expression of gibberellin-related genes （P<0.05）. In summary， the effects of uniconazole and paclobutrazol on the growth and gene expression of P. pratensis were opposite to those of gibberellin， but there was no significant concentration effect. The growth inhibition from spraying uniconazole or paclobutrazol diluted 1000 times after lawn mowing would have significantly reduced the labor cost of lawn maintenance ， enhanced lawn visual appearance and ornamental value and promoted ease of management and turf persistence in landscaping applications. |
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| Keywords: | Poa pratensis growth regulator gibberellin growth gene expression |
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