| 基于AFLP标记的河八王种质资源遗传多样性分析及分子身份证构建 |
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| 引用本文： | 刘昔辉,张荣华,张革民,周会,宋焕忠,方位宽,区惠平,杨丽涛,李扬瑞.基于AFLP标记的河八王种质资源遗传多样性分析及分子身份证构建[J].华中农业大学学报,2016,35(4):7-13. |
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| 作者姓名： | 刘昔辉 张荣华 张革民 周会 宋焕忠 方位宽 区惠平 杨丽涛 李扬瑞 |
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| 作者单位： | 1. 广西大学农学院,南宁530004;广西甘蔗遗传改良重点实验室/中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术与遗传改良重点实验室,南宁530007;2. 广西甘蔗遗传改良重点实验室/中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术与遗传改良重点实验室,南宁,530007 |
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| 基金项目： | “863”计划项目(2013AA102604)；国家自然科学基金项目(31101195, 31360357)；国际合作项目(2013DFA31600)； 广西基金项目(2012GXNSFBA053048,2014GXNSFBA118118,桂科合14123001 1 4)； 广西农科院基金项目(桂农科2012YZ10, 2013JQ14)；广西“八桂学者”和特聘专家专项(2013) |
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| 摘 要： | 以广西地区的15份河八王无性系为材料,采用AFLP标记结合毛细管电泳进行分析,计算多态性引物的总扩增条带数、多态性条带数和多态性条带比率。进行遗传相似系数和UPGMA聚类分析,并建立分子身份证。25对AFLP引物组合在15份河八王种质资源中共扩增出2 208个位点,其中多态性位点1 914个,多态性比率(PPB)为87.11%。UPGMA聚类分析表明,供试的15份河八王种质资源其遗传相似系数变化范围在0.656~0.878,在相似系数为0.706时,可划分为3个类群。结合特征带和不同引物组合方法可有效建立河八王种质资源特异分子身份证,置信概率达到99.99%。所供试河八王种质具有较丰富的遗传多样性水平,基于3对AFLP引物组合104条谱带构建的15份河八王种质分子身份证具有唯一性,AFLP标记是在分子水平上鉴定河八王种质的有效方法。
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| 关 键 词： | 河八王 种质资源 遗传多样性 AFLP标记 分子身份证 |
| 收稿时间： | 2016/3/31 0:00:00 |
Genetic diversity and molecular identification card construction of Narenga porphyrocoma (Hance) Bor germplasm based on AFLP markers |
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| Abstract: | The effects of basal medium and explants on the primary culture of dormant buds of date plum (Diospyros lotus Linn.) were studied.The effects of inoculation methods,TDZ concentrations,combinations of plant growth regulators and induction period under dark condition on callus formation,adventitious bud regeneration and root regeneration were investigated.The results showed that DKW was the best basal medium for the primary culture of dormant buds,as revealed by better growth of the buds.Genotypes had no impacts on primary culture,but influenced subculture obviously.Inoculation of the segments with the adaxial surfaces contacting medium on MS+ZT 0.1 mg/L+TDZ 2.0 mg/L under darkness for 20 d led to 100% formation of callus,but the percentage was only 81.4% on (1/2N)MS+2.200 mg/L TDZ.Inoculation of leaf segments,adaxial surfaces contacting medium,on MS medium containing ZT 1.0 mg/L+TDZ 2.0 mg/L,dark treatment for 0 d,resulted in the 34.4% of shoot regeneration,while average shoots numbers per explant were 2.1±0.3.Inoculated the plantlets on 1/2MS(1/2N)+IBA 1.0 mg/L for 5 d,then transplanted them on 1/2MS(1/2N)+1 g/L activated carbon for 20 d.Rooting of date plum reached 58.14% and the average number of adventitious roots was 4. |
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| Keywords: | Narenga porphyrocoma (Hance) Bor germplasm genetic diversity AFLP marker molecular identification card |
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