Genetic control of somatic embryogenesis in alfalfa (Medicago sativa L. cv. Adriana) |
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| Authors: | F Crea M Bellucci F Damiani S Arcioni |
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| Institution: | (1) Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del C.N.R., Via Madonna Alta 130, 06128 Perugia, Italy |
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| Abstract: | Summary In alfalfa (Medicago sativa) regeneration is genotype-specific. In order to study the genetic control of somatic embryogenesis and to constitute a synthetic cultivar characterized by its high regeneration ability, 2 embryogenic plants selected from the cv. Adriana were selfed, intercrossed and also crossed in both directions with 5 non-embryogenic genotypes of the same cultivar.Progenies of all crosses were scored for their regeneration ability and results indicate that somatic embryogenesis is under the control of 2 dominant loci. However some non-embryogenic genotypes prevent regeneration when crossed with embryogenic ones and this characteristic is not under the control of a single dominant gene.When plants chosen for their capacity to regenerate within F1 and S1 progenies were freely intercrossed the regeneration efficiency dropped to 2% (1 plant out of 50). This result indicates that if the genetic background of the population is changed the regeneration is greatly affected and therefore some other mechanism could play a role in determining plant regeneration. |
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| Keywords: | Medicago sativa somatic embryogenesis genetic control |
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